Phytochemical profiling of several Hypericum species identified using genetic markers. (July 2021)

• Record Type: Journal Article
• Authors: Bruňáková, Katarína; Bálintová, Miroslava; Henzelyová, Jana; Kolarčik, Vladislav; Kimáková, Andrea; Petijová, Linda; Čellárová, Eva
• Is Part Of: Phytochemistry. Volume 187(2021)
• Publication Date: 2021-07
• Subjects: Hypericum spp. -- Hypericaceae -- DNA barcoding -- Flow cytometry -- Chromosome counting -- HPLC -- Naphthodianthrones -- Phloroglucinols -- Flavonoids; Botanical chemistry -- Periodicals; Biochemistry -- Periodicals; Botany -- Periodicals; Chimie végétale -- Périodiques; 572.2
• DOI: 10.1016/j.phytochem.2021.112742
• Languages: English
• ISSNs: 0031-9422

Abstract

Abstract: In the present study, we performed phytochemical profiling of several under-exploited Hypericum representatives taxonomically belonging to the sections Ascyreia, Androsaemum, Inodora, Hypericum, Coridium, Myriandra, and Adenosepalum . The authenticity of the starting plant material was confirmed using the nuclear ribosomal internal transcribed spacer as a molecular marker, DNA content and chromosome number. Phenolic constituents were analyzed using high-performance liquid chromatography to complement species-specific metabolic profiles. In several Hypericum representatives, the pharmacologically important compounds, including naphthodianthrones; phloroglucinol derivatives; chlorogenic acid; and some classes of flavonoids, particularly the flavonols rutin and hyperoside, flavanol catechin, and flavanones naringenin and naringin, were reported for the first time. Comparative multivariate analysis of chemometric data for seedlings cultured in vitro and acclimated to the outdoor conditions revealed a strong genetically predetermined interspecific variability in phenolic compound content. In addition to hypericins, which are the most abundant chemomarkers for the genus Hypericum, rarely employed phenolic metabolites, including phloroglucinol derivatives, chlorogenic acid, catechin, naringenin, naringin, and kaempferol-3-O-glucoside, were shown to be useful for discriminating between closely related species. Given the increasing interest in natural products of the genus Hypericum, knowledge of the spectrum of phenolic compounds in shoot cultures is a prerequisite for future biotechnological applications. In addition, phytochemical profiling should be considered as an additional part of the integrated plant authentication system, which predominantly relies upon genetic markers. Graphical abstract: Image 1 Highlights: Phytochemical profiling of some under-exploited Hypericum species was performed. Integrated authentication with genetic markers and phytochemical profiles is required. Genetic identifiers include ITS barcode, DNA content, and ploidy level. Multivariate analysis revealed reliable metabolites for species clustering. Phenolic spectrum is predominantly determined by genetic predisposition.