Thin cell layer cultures of Chlamydomonas reinhardtii L159I-N230Y, pgrl1 and pgr5 mutants perform enhanced hydrogen production at sunlight intensity. (August 2021)

• Record Type: Journal Article
• Authors: Nagy, Valéria; Podmaniczki, Anna; Vidal-Meireles, André; Kuntam, Soujanya; Herman, Éva; Kovács, László; Tóth, Dávid; Scoma, Alberto; Tóth, Szilvia Z.
• Is Part Of: Bioresource technology. Volume 333(2021)
• Publication Date: 2021-08
• Subjects: Chlamydomonas reinhardtii -- Biohydrogen -- Hydrogenase -- Photosynthesis -- Thin cell layer cultures; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2021.125217
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Anaerobiosis-induced photoautotrophic H2 production lasts days in continuous light. Dense thin-layer cultures have improved H2 production at sunlight intensity. H2 productivities of the L159I-N230Y, pgrl1, and pgr5 mutants are enhanced. Abstract: Photobiological hydrogen (H2 ) production is a promising renewable energy source. HydA hydrogenases of green algae are efficient but O2 -sensitive and compete for electrons with CO2 -fixation. Recently, we established a photoautotrophic H2 production system based on anaerobic induction, where the Calvin-Benson cycle is inactive and O2 scavenged by an absorbent. Here, we employed thin layer cultures, resulting in a three-fold increase in H2 production relative to bulk CC-124 cultures (50 µg chlorophyll/ml, 350 µmol photons m −2 s −1 ). Productivity was maintained when increasing the light intensity to 1000 µmol photons m -2 s −1 and the cell density to 150 µg chlorophyll/ml. Remarkably, the L159I-N230Y photosystem II mutant and the pgrl1 photosystem I cyclic electron transport mutant produced 50% more H2 than CC-124, while the pgr5 mutant generated 250% more (1.2 ml H2 /ml culture in six days). The photosynthetic apparatus of the pgr5 mutant and its in vitro HydA activity remained remarkably stable.