The association of tumor necrosis factor alpha, lymphotoxin alpha, tumor necrosis factor receptor 1 and tumor necrosis factor receptor 2 gene polymorphisms and serum levels with periodontitis and type 2 diabetes in Serbian population. (December 2020)

• Record Type: Journal Article
• Authors: Petrovic, Sanja Matic; Nikolic, Nadja; Toljic, Bosko; Arambasic-Jovanovic, Jelena; Milicic, Biljana; Milicic, Tanja; Jotic, Aleksandra; Vidakovic, Melita; Milasin, Jelena; Pucar, Ana
• Is Part Of: Archives of oral biology. Volume 120(2020)
• Publication Date: 2020-12
• Subjects: PD periodontitis -- T2D type 2 diabetes -- TNFα tumor necrosis factor-alpha -- LTα lymphotoxin-alpha -- TNFR1 tumor necrosis factor receptor 1 TNFR2 tumor necrosis factor receptor 2 -- sTNFα soluble TNFα -- sLTα soluble LTα -- sTNFR1 soluble TNFR1 -- sTNFR2 soluble TNFR2 -- SNP single nucleotide polymorphism -- PCR-RFLP polymerase chain reaction- restriction fragment length polymorphism -- ELISA enzyme-linked immunosorbent assay -- PESA periodontal epithelial surface area -- PISA periodontal inflamed surface area -- BMI body mass index -- PY pack-years -- PI plaque index -- BOP bleeding on probing -- PPD probing pocket depth -- CAL clinical attachment loss -- GR gingival recession -- OGTT oral glucose tolerance test -- HbA1c glycated hemoglobin -- FPG fasting plasma glucose -- HWE Hardy-Weinberg equilibrium -- OR odds ratio -- CI confidence interval -- R2 determination coefficient -- IQR interquartile range; Tumor necrosis factor - alpha -- Single nucleotide polymorphisms -- Periodontitis -- Type 2 diabetes mellitus -- Tumor necrosis factor receptors; Mouth -- Periodicals; Mouth -- Diseases -- Periodicals; Dentistry -- Periodicals; Electronic journals; 617.6005
• DOI: 10.1016/j.archoralbio.2020.104929
• Languages: English
• ISSNs: 0003-9969

Abstract

Highlights: +252A/G LTα and +676 T/G TNFR2 SNP are connected toincreased/decreased risk for PD. Periodontal destruction may be influenced by +36 A/G TNFR1 SNP in healthy subjects. PESA and PISA correlated negatively with sTNFR2 in healthy subject. Influence of periodontal on systematic inflammation may be masked by T2D. Periodontal destruction in diabetics is probably a consequence of T2D. Abstract: Objectives: Aiming to show that periodontitis (PD) and type 2 diabetes (T2D) are bidirectionally related and potentially linked by inflammatory cytokines, we searched for association between −308 G/A Tumor necrosis factor-alpha (TNFα), +252A/G Lymphotoxin-alpha (LTα), +36A/G Tumor necrosis factor receptor 1 (TNFR1) and +676 T/G tumor necrosis factor receptor 2 (TNFR2) single nucleotide polymorphisms (SNPs) and: risk of PD or PD + T2D; periodontitis parameters in PD and PD + T2D; serum levels of cytokines/their receptors. Relationship between periodontal inflammation and serum cytokine/receptor levels was also assessed. Design: Subjects were stratified as: 57 healthy controls (HC); 58 PD; 65 PD + T2D. Sociodemographic, environmental, behavioral and periodontal clinical data were recorded. SNPs were genotyped using polymerase chain reaction-restriction fragment length polymorphism, while cytokines/receptors levels were quantified by enzyme-linked immunosorbent assay. Impact of periodontal inflammation was measured using periodontal inflamed surface area (PISA). Results: TNFα AA genotype showed protective effect in T2D + PD compared to PD, even adjusted for behavioral/environmental factors (OR 0.18; 95 %CI 0.037−0.886; p = 0.035). LTα AG heterozygotes had increased risk of PD (OR 3.27; 95 %CI 1.35−7.96; p = 0.016), while TNFR2 TG genotype had protective effect (OR = 0.44; 95 %CI 0.954−0.9794; p = 0.043). TNFR1 AA was predictor of periodontal pocket depth and clinical attachment loss in PD. Correlation between TNFR2 concentration and PISA was negative in PD, positive in PD + T2D. Conclusions: None of the SNPs showed cross-susceptibility between PD and T2D. + 252A/G LTα and +676 T/G TNFR2 SNPs are associated with PD risk. Periodontal destruction in healthy individuals is influenced by TNFR1 genotype. Impact of periodontal on systemic inflammation is masked by T2D.