Physicochemical impact of bioactive terpenes on the microalgae biomass structural characteristics. (August 2021)

• Record Type: Journal Article
• Authors: Gouda, Mostafa; Huang, Zhenxiong; Liu, Yufei; He, Yong; Li, Xiaoli
• Is Part Of: Bioresource technology. Volume 334(2021)
• Publication Date: 2021-08
• Subjects: Microalgae biomass -- Functional properties -- Bioactive terpenes -- Bioresource technology -- Protein folding; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2021.125232
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Vanillin (0.1%) increased Arthrospira platensis secondary amines Raman peak at 1198 cm −1 . Chlorella vulgaris viscosity was significantly increased by 0.1% trans -cinnamaldehyde. This study multi-combined techniques confirmed the mechanism analysis of microalgae changes. Arthrospira platensis α-helix decreased significantly by 0.1% Vanillin. Surface hydrophobicity (Ho) was positivity correlated (r 2 = 0.91) with micro-Raman at 1196 cm −1 . Abstract: This study aimed to evaluate the functionality of bioactive terpenes on Spirulina ( Arthrospira platensis ; AP) and Chlorella ( Chlorella vulgaris ; CV) biomasses. The two microalgae species were treated with 0.01%, 0.05%, and 0.1% of thymol (THY), trans -cinnamaldehyde (TC), menthol (MEN), and vanillin (VAN). Raman micro-spectroscopy (RMS) was correlated with other physicochemical methods to confirm their functional mechanisms. In results, THY (0.1%) decreased ( P < 0.05) RMS intensity at 1196 cm −1 that represents the protein's secondary amines wavenumber. Also, VAN (0.1%) decreased significantly A. platensis α-helix to 16.60 ± 0.52% compared to the control with 19.83 ± 0.32%. While, 0.1% TC increased ( P < 0.05) the viscosity to 2.52 ± 0.61 Pa.s. This work demonstrated that terpenes could differently affect the physicochemical structure of microalgae biomass. The RMS's uniqueness comes from its ability to evaluate the functionality of terpenes during microalgae cultivation. Besides, chemometrics led to focus on the most important variances.