Molecular structures of nonvolatile components in the Haihong fruit wine and their free radical scavenging effect. (15th August 2021) - Hui, Yang
Highlights: The radical scavenging effect of flavonoids is closely related to the structure of diphenol. The electron conjugation in reactant is a key factor for free radical scavenging. The chemical shift of phenol hydrogen is a measure of radical scavenging ability. Haihong fruit wine can enhance the activities of SOD, CAT and GPX in mice liver. Abstract: In order to investigate the correlation between free radical scavenging effect and the related molecular structures of active substances in the Haihong fruit ( Malus Micromalus Makino ) wine, sixteen kinds of components were isolated from the fruit wine. The structures of thirteen components were identified by UV, FTIR, LC-MS, 1D-NMR and 2D-NMR. The scavenging abilities of the fruit wine on DPPH (2, 2-Diphenyl-1 -picrylhydrazyl radical), OH, O2 − and the protective effects on red blood cell, SOD (Superoxide Dismutase), CAT(Catalase) and GPX(glutathione peroxidases) in aging mice tissues were studied. Results showed that the structures of o -diphenol and m -diphenol play an important role in scavenging free radicals. A larger conjugation system in functional molecule is conducive to getting a higher scavenging rate of free radicals. When the chemical shift of phenol hydrogen is lower, the anti-oxygenation ability is stronger. The fruit wine exhibits a strong scavenging ability on free radicals. It can inhibit the damage of red blood cells caused by OH radical.