Microsatellites Cross-Species Amplification across Some African Cichlids. (4th June 2012)

• Record Type: Journal Article
• Authors: Bezault, Etienne; Rognon, Xavier; Gharbi, Karim; Baroiller, Jean-Francois; Chevassus, Bernard
• Other Names: Sefc Kristina M. Academic Editor.
• Is Part Of: International journal of evolutionary biology. Volume 2012(2012)
• Publication Date: 2012-06-04
• Subjects: Evolution (Biology) -- Periodicals; Biological Evolution; Biological Phenomena; Evolution (Biology); Electronic journals; Periodical; Fulltext; Internet Resources; Periodicals; Periodicals; 576.805
• DOI: 10.1155/2012/870935
• Languages: English
• ISSNs: 2090-8032

Abstract

Abstract : The transfer of the genomic resources developed in the Nile tilapia, Oreochromis niloticus, to other Tilapiines sensu lato and African cichlid would provide new possibilities to study this amazing group from genetics, ecology, evolution, aquaculture, and conservation point of view. We tested the cross-species amplification of 32 O. niloticus microsatellite markers in a panel of 15 species from 5 different African cichlid tribes: Oreochromines ( Oreochromis, Sarotherodon ), Boreotilapiines ( Tilapia ), Chromidotilapines, Hemichromines, and Haplochromines. Amplification was successfully observed for 29 markers (91%), with a frequency of polymorphic (P95 ) loci per species around 70%. The mean number of alleles per locus and species was 3.2 but varied from 3.7 within Oreochromis species to 1.6 within the nontilapia species. The high level of cross-species amplification and polymorphism of the microsatellite markers tested in this study provides powerful tools for a wide range of molecular genetic studies within tilapia species as well as for other African cichlids.