Novel Implant Coating Agent Promotes Gene Expression of Osteogenic Markers in Rats during Early Osseointegration. (30th October 2012)

• Record Type: Journal Article
• Authors: Bougas, Kostas; Jimbo, Ryo; Xue, Ying; Mustafa, Kamal; Wennerberg, Ann
• Other Names: Elias Carlos Nelson Academic Editor.
• Is Part Of: International journal of biomaterials. Volume 2012(2012)
• Publication Date: 2012-10-30
• Subjects: Biomedical materials -- Periodicals; Biomedical and Dental Materials; Biomedical materials; Electronic journals; Periodicals; Fulltext; Internet Resources; Periodicals; Periodicals; 610.28
• DOI: 10.1155/2012/579274
• Languages: English
• ISSNs: 1687-8787

Abstract

Abstract : The aim of this study was to evaluate the early bone response around laminin-1-coated titanium implants. Forty-five rats distributed in three equally sized groups were provided with one control (turned) and one test (laminin-1-coated) implant and were sacrificed after 3, 7, and 21 days. Real-time reverse-transcriptase polymerase chain reaction was performed for osteoblast markers (alkaline phosphatase, runt-related transcription factor 2, osteocalcin, type I collagen, and bone morphogenic protein 2), osteoclast markers (cathepsin K and tartrate-resistant acid phosphatase), inflammation markers (tumor necrosis factor α, interleukin 1 β and interleukin 10), and integrin β 1. Bone implant contact (BIC) and bone area (BA) were assessed and compared to the gene expression. After 3 days, the expression of bone markers was higher for the control group. After 7 days, the expression of integrin β 1 and osteogenic markers was enhanced for the test group, while cathepsin K and inflammation markers were down-regulated. No significant differences in BIC or BA were detected between test and control at any time point. As a conclusion, implant coating with laminin-1 altered gene expression in the bone-implant interface. However, traditional evaluation methods, as histomorphometry, were not adequately sensitive to detect such changes due to the short follow-up time.