Investigation of Bacterial Cellulose Biosynthesis Mechanism in Gluconoacetobacter hansenii. (15th March 2014)

• Record Type: Journal Article
• Authors: Mohite, Bhavna V.; Patil, Satish V.
• Other Names: Ruiz-Herrera J. Academic Editor.; West T. P. Academic Editor.
• Is Part Of: ISRN microbiology. Volume 2014(2014)
• Publication Date: 2014-03-15
• Subjects: Microbiology -- Periodicals; Microbiology; Microbiological Phenomena; Fulltext; Internet Resources; Periodicals; 579
• DOI: 10.1155/2014/836083
• Languages: English
• ISSNs: 2090-7478

Abstract

Abstract : The present study explores the mechanism of cellulose biosynthesis in Gluconoacetobacter hansenii . The cellulose synthase enzyme was purified as membrane fraction and solubilized by treatment with 0.1% digitonin. The enzyme was separated by native-gel electrophoresis and β -D-glucan analysis was carried out using in vitro gel assay. The cellulose synthase has glycoprotein nature and composed two polypeptide subunits of 93 KDa and 85 KDa. The confirmation of β -1, 4-glucan (cellulose) was performed in whole and hydrolyzed monomeric sugar form. Tinopal and Congo red were used for cellulose detection on the gel. Thus the in vitro cellulose synthesis assay with cell free enzyme fraction was attempted to improve the understanding of cellulose biosynthesis.