Genome-wide DNA methylation profiling of high-fat emulsion-induced hyperlipidemia mice intervened by a polysaccharide from Cyclocarya paliurus (Batal) Iljinskaja. (June 2021)

• Record Type: Journal Article
• Authors: Yang, Zhanwei; Zhao, Jing; Li, Jing'en; Wang, Jin; Wang, Wenjun
• Is Part Of: Food and chemical toxicology. Volume 152(2021)
• Publication Date: 2021-06
• Subjects: Conjoint analysis -- Cyclocarya paliurus (Batal) iljinskaja -- Hyperlipidemia -- Lipid metabolism -- Signaling pathway; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2021.112230
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: Genome-wide DNA methylation was used to study the lipid-lowering effect of Cyclocarya paliurus (Batal) Iljinskaja polysaccharide (CPP). The objective of this study was to investigate the hypolipidemic effects and the potential underlying mechanisms of action of CPP-2 in high-fat emulsion (HFE)-induced mice. The results showed that CPP-2 reduced the level of genome-wide DNA methylation in the liver of HFE-induced mice, which had a lipid-lowering effect by regulating the AMP-activated protein kinase (AMPK) signaling-, fatty acid metabolism-, fatty acid biosynthesis- and adipocytokine signaling pathways. A series of lipid metabolism genes were screened out by conjoint analysis of the Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Hereafter, fatty acid synthase (FAS) and peroxisome proliferators-activated receptor α (PPARα) as target genes were selected to validate the accuracy of the results. The findings demonstrated that CPP-2 might be effective in lowering the lipid content, thereby protecting against HFE-induced hyperlipidemia. Graphical abstract: Image 1 Highlights: Genome-wide DNA methylation profiling was used to study the hypolipidemic effect of CPP-2. CPP-2 regulated lipid metabolism by AMPK and fatty acid metabolism pathways. Conjoint analysis of GO and KEGG was used to screen for lipid metabolism genes.