Seroprevalence of SARS-CoV-2 in blood donors from the Lodi Red Zone and adjacent Lodi metropolitan and suburban area. (June 2021)

• Record Type: Journal Article
• Authors: Cassaniti, Irene; Percivalle, Elena; Sarasini, Antonella; Cambiè, Giuseppe; Vecchio Nepita, Edoardo; Maserati, Roberta; Ferrari, Alessandro; Corcione, Alfonso; Di Martino, Raffaella; Bonetti, Alice; Di Napoli, Annapia; Ferrari, Guglielmo; Baldanti, Fausto
• Is Part Of: Clinical microbiology and infection. Volume 27:Number 6(2021)
• Page Start: 914.e1
• Page End: 914.e4
• Publication Date: 2021-06
• Subjects: Epidemiology -- Lodi area -- Microneutralization assay -- Serological assay -- Seroprevalence -- Severe acute respiratory syndrome coronavirus 2; Medical microbiology -- Periodicals; Diagnostic microbiology -- Periodicals; Communicable diseases -- Periodicals; Infection -- Periodicals; 616.01
• DOI: 10.1016/j.cmi.2021.01.030
• Languages: English
• ISSNs: 1198-743X

Abstract

Abstract: Objectives: To define the seroprevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in blood donors (referred to the first lockdown area (Lodi Red-Zone) of the Lombardy region and in a contiguous area that was not included in the first lockdown); to define the agreement between a commercial serological assay and a reference microneutralization assay; and to evaluate the persistence of SARS-CoV-2 neutralizing antibodies in a cohort of blood donors. Methods: Blood donors referred to the first lockdown area in Lombardy Region and the neighbouring area were analysed for SARS-CoV-2 IgG-specific antibodies during the period 18 March to 24 June 2020. Serum samples were analysed using both a chemiluminescent immunoassay (LIAISON® SARS-CoV-2 S1/S2 IgG, DiaSorin) for the quantitative characterization of SARS-CoV-2 anti-S1 and anti-S2 IgG antibodies and a neutralizing antibodies (NT-Abs) assay. Results: In the period from 18 March to 24 June, 1922 blood donors were tested for the presence of SARS-CoV-2 IgG showing a prevalence of 378/1922 (19.7%). A subgroup of 1139 blood donors were tested in parallel with a SARS-CoV-2 IgG assay and a microneutralization assay showing a prevalence of 22.2% and 21.6%, respectively. SARS-CoV-2 IgG quantification was correlated with NT-Abs titres. In 78.2% of participants the NT-Abs titre was maintained, but in 15.8% it decreased by one four-fold dilution and in 6.0% it increased by one four-fold dilution. Conclusions: The duration of immunity of SARS-CoV-2 is crucial for the course of the pandemic and for this reason the monitoring of NT Abs is important. Despite a stable NT-Abs titre being observed in the majority of blood donors, our findings need to be validated in a long-term period of follow up.