The protective effects of curcumin in cerebral ischemia and reperfusion injury through PKC-θ signaling. Issue 5 (19th March 2021)

• Record Type: Journal Article
• Authors: Mo, Yun; Yue, Erli; Shi, Nan; Liu, Kangyong
• Is Part Of: Cell cycle. Volume 20:Issue 5/6(2021)
• Page Start: 550
• Page End: 560
• Publication Date: 2021-03-19
• Subjects: Curcumin -- OGD/R -- PKC-θ; Cell cycle -- Periodicals; 571.84377
• DOI: 10.1080/15384101.2021.1889188
• Languages: English
• ISSNs: 1538-4101

Abstract

ABSTRACT: Ischemic stroke is a common cerebrovascular disease with the main cause considered to be cerebral ischemia and reperfusion (I/R), which exerts irreparable injury on nerve cells. Thus, the development of neuroprotective drugs is an urgent concern. Curcumin, a known antioxidant, has been found to have neuroprotective effects. To determine the protective mechanism of curcumin in ischemic stroke, oxygen and glucose deprivation/reoxygenation (OGD/R) was used to treat PC12 cells to mimic the cerebral I/R cell model. Curcumin (20 μM) was applied to OGD/R PC12 cells, followed by Ca 2+ concentration, transepithelial electrical resistance (TEER), and cell permeability measurements. The results showed that OGD/R injury induced a decrease in TEER and increases in Ca 2+ concentration and cell permeability. In contrast, curcumin alleviated these effects. The protein kinase C θ (PKC-θ) was associated with the protective function of curcumin in the OGD/R cell model. Moreover, the middle cerebral artery occlusion and reperfusion model (MCAO/R) was applied to simulate the I/R rat model. Our results demonstrated that curcumin could reverse the MCAO/R-induced increase in Ca 2+ concentration and blood–brain barrier (BBB) disruption. Our study demonstrates the mechanisms by which curcumin exhibited a protective function against cerebral I/R through PKC-θ signaling by reducing BBB dysfunction.