Oxidative stress in response to heat stress in wild caught Namaqua rock mice, Micaelamys namaquensis. (May 2021)

• Record Type: Journal Article
• Authors: Jacobs, Paul J.; Oosthuizen, M.K.; Mitchell, C.; Blount, J.D.; Bennett, N.C.
• Is Part Of: Journal of thermal biology. Volume 98(2021)
• Publication Date: 2021-05
• Subjects: Oxidative stress -- Dehydration -- Thermal stress -- Climate change -- Micaelamys namaquensis; Thermobiology -- Periodicals; Temperature -- Periodicals; Biology -- Periodicals; Thermobiologie -- Périodiques; Thermobiology; Periodicals; 571.46
• DOI: 10.1016/j.jtherbio.2021.102958
• Languages: English
• ISSNs: 0306-4565

Abstract

Abstract: Modelling of anthropogenic induced climate suggests more frequent and severe heatwaves in the future, which are likely to result in the mass die-off of several species of organisms. Oxidative stress induced by severe heat stress has previously been associated with a reduction in animal cognitive performance, depressed reproduction and lower life expectancy. Little is known about the non-lethal consequences of species should they survive extreme heat exposure. We investigated the oxidative stress experienced by the Namaqua rock mouse, a nocturnal rodent, using two experimental heat stress protocols, a 6 hour acute heat stress protocol without access to water and a 3-day heatwave simulation with ad libitum water. Oxidative stress was determined in the liver, kidney and brain using malondialdehyde (MDA) and protein carbonyl (PC) as markers of oxidative damage, and superoxide dismutase (SOD) and total antioxidant capacity (TAC) as markers of antioxidant defence. Incubator heat stress (heat and dehydration stress) was brought about by increasing the body temperatures of animals to 39–40.8 °C for 6 hours. Following incubator heat stress, significantly higher levels of MDA were observed in the liver. Dehydration did not explain the variation in oxidative markers and is likely a combined effect of thermal and dehydration stress. Individual body mass was significantly negatively correlated to kidney SOD and lipid peroxidation. A heatwave was simulated using a temperature cycle that would naturally occur during a heatwave in the species' local habitat, with a maximal ambient temperature of 38 °C. Following the simulated heatwave, SOD activity of the kidney demonstrated significantly lowered activity suggesting oxidative stress. Current heat waves in this species have the potential of causing oxidative stress. Heat and dehydration stress following exacerbated temperatures are likely to incur significant oxidative stress in multiple tissues demonstrating the importance of water availability to allow for rehydration to prevent oxidative stress. Highlights: Heat and dehydration resulted in increased lipid peroxidation in the liver. Reduced superoxide dismutase activity in the kidney followed a simulated heatwave. Superoxide dismutase and lipid peroxidation correlated with body mass for the kidney.