Key taste components in two wild edible Boletus mushrooms using widely targeted metabolomics. (June 2021)

• Record Type: Journal Article
• Authors: Li, Jinglin; Wu, Haiying; Wang, Li; Huang, Yongjie; Wang, Linbo
• Is Part Of: Biochemical systematics and ecology. Volume 96(2021)
• Publication Date: 2021-06
• Subjects: Boletus edulis -- Metabolites -- PCA -- OPLS-DA -- UPLC-ESI-MS/MS -- QQQ; ESI electrospray ionization -- LIT linear ion trap -- LC-MS/MS liquid chromatography-tandem mass spectrometry -- OPLS-DA orthogonal signal correction and partial least squares-discriminant analysis -- PCA principal component analysis -- QQQ triple quadrupole -- Q TRAP quadrupole-linear ion trap mass spectrometer -- UPLC-ESI-MS/MS ultra-performance liquid chromatography-electrospray ionization-tandem mass spectrometry -- VIP variable importance in projection; Chemotaxonomy -- Periodicals; Biochemical variation -- Periodicals; Ecology -- Periodicals; Biochemistry -- Periodicals; Ecology -- Periodicals; Chimiotaxinomie -- Périodiques; Variation biochimique -- Périodiques; Écologie -- Périodiques; 578.012
• DOI: 10.1016/j.bse.2021.104268
• Languages: English
• ISSNs: 0305-1978

Abstract

Abstract: Compounds from wild edible mushrooms has been reported to exert biological activities and contribute to the different flavors of mushrooms in our diet. Wild edible Boletus mushrooms are popular in Southwest China. In this study, we performed ultra-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS) based on widely targeted metabolomics analysis to identify key components. A total of 194 metabolites (113 upregulated, 81 downregulated) divided into 11 groups (49 metabolites in group lipids, 34 in amino acids and derivatives, 30 in organic acids, 18 in phenolic acids, 16 in nucleotides and derivatives, 13 in alkaloids, 6 in flavonoids, 3 in lignans and coumarins, 3 in tannins, 2 in terpenoids, and 20 in others) were found among Boletus bainiugan compared with B. subsplendidus . Through clustering analysis, principal component analysis (PCA), and orthogonal signal correction and partial least squares-discriminant analysis (OPLS-DA), different metabolites from fruiting bodies were clearly identified. Significant differences were observed in the metabolites between Boletus bainiugan and B. subsplendidus . These metabolites are involved in important biological functions. Our results provide new insights into important metabolites and theoretical basis for the taste difference of two wild edible Boletus mushrooms. Highlights: Wild edible mushrooms exert biological activities and different taste. Using UPLC-ESI-MS/MS based on widely targeted metabolomics to identify key components of wild edible Boletus mushrooms. New insights into important metabolites among the two mushrooms. Concentrations of organic acids, amino acids, and lipids compounds may discover difference in taste.