Clinical significance of serum miR-487b in HBV-related hepatocellular carcinoma and its potential mechanism. Issue 7 (3rd July 2021)

• Record Type: Journal Article
• Authors: Li, Xiuping; Guo, Yuzhen; Wang, Xiyan; Ge, Anning; Wang, Hui; Fan, Kaiyun; Guo, Changtong
• Is Part Of: Infectious diseases. Volume 53:Issue 7(2021)
• Page Start: 546
• Page End: 554
• Publication Date: 2021-07-03
• Subjects: Hepatocellular carcinoma -- hepatitis B virus -- microRNA-487b -- diagnostic -- prognostic; Communicable diseases -- Periodicals; Communicable Diseases -- Periodicals; Communicable diseases; Periodicals; 616.9
• DOI: 10.1080/23744235.2021.1901981
• Languages: English
• ISSNs: 2374-4235

Abstract

Abstract: Background: Hepatocellular carcinoma (HCC) is a malignant tumour with high mortality. In recent years, microRNA (miRNA) has been recognized in the diagnosis and prognosis of cancer. miR-487b has been found to play a role in a variety of cancers. The purpose of this study is to explore the role of miR-487b in the diagnosis and prognosis of hepatitis B virus (HBV)-related HCC, and its influence on the biological behaviour of HCC cells. Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) method was used to detect the expression level of miR-487b in the serum of HCC patients, HBV patients, and healthy people. The ROC curve was used to evaluate the role of miR-487b in the diagnosis of HCC. The prognostic significance of miR-487b in HCC was analyzed by the Kaplan-Meier survival curve and Cox regression model. The effects of miR-487b on cell proliferation, migration, and invasion were explored through MTT assay and transwell assays. Results: The expression level of miR-487b in the serum of HBV-related HCC patients was significantly higher than that of CHB patients and normal healthy people. The expression level of miR-487b can distinguish HCC patients from CHB patients or normal healthy people. High expression of miR-487b was associated with poor prognosis, which could be used as an independent prognostic factor for HCC. The upregulation of miR-487b promoted cell proliferation, migration, and invasion. Conclusion: miR-487b can be used as a biomarker for the diagnosis and prognosis of HCC.