Using nanoselenium to combat Minamata disease in rats: the regulation of gut microbes. Issue 5 (22nd April 2021)

• Record Type: Journal Article
• Authors: Liu, Yang; Zhang, Wei; Zhao, Jiating; Lin, Xiaoying; Wang, Liming; Cui, Liwei; Zhang, Junfang; Li, Bai; Li, Yu-Feng
• Is Part Of: Environmental science. Volume 8:Issue 5(2021)
• Page Start: 1437
• Page End: 1445
• Publication Date: 2021-04-22
• Subjects: Environmental sciences -- Periodicals; Nanotechnology -- Periodicals; 620.505
• DOI: 10.1039/d1en00267h
• Languages: English
• ISSNs: 2051-8153

Abstract

Abstract : Nanoselenium promoted demethylation of methylmercury and regulated gut microbes in methylmercury-poisoned rats. Abstract : Methylmercury (MeHg) can magnify through food chains and cause potent neurological problems like Minamata disease. Gut microbes have been found to demethylate MeHg while MeHg can destroy the diversity of gut microbes. Our recent study found that selenite (Se 4+ ) could promote the demethylation of MeHg and regulate the diversity of gut microbes in MeHg-poisoned rats (Minamata disease). Elemental selenium at nano-size (nanoSe) was less toxic than Se 4+ with comparable bioavailability. In this study, nanoSe was studied on its demethylation of MeHg and regulation of gut microbes in rats with Minamata disease using Se 4+ as a positive control. Rats with Minamata disease were treated with nanoSe or Se 4+ every other day for 90 days. Fecal samples were collected on days 8, 30, 60 and 90, and concentrations of Se, Hg and MeHg were measured. The diversity and abundance of gut microbes were determined using 16S rRNA gene profiling. It was found that both nanoSe and Se 4+ enhanced the demethylation of MeHg, especially before day 30. The percentage of MeHg (of total mercury) in the MeHg-poisoned group was in the range of 81–105% while it was 23–79% in the nanoSe group and 65–84% in the Se 4+ group, suggesting the higher demethylation rate of nanoSe than that of Se 4+ . Both nanoSe and Se 4+ regulated the diversity and abundance of gut microbes at both the phylum and genus rank. Therefore, considering its low toxicity, nanoSe is more desirable in combating Minamata disease. Besides, this study confirmed the new role of Se against MeHg poisoning, i.e. the regulation of gut microbes.