Binding of the Bi (III) Complex of Naringin with β-Cyclodextrin/Calf Thymus DNA: Absorption and Fluorescence Characteristics. (5th June 2014)

• Record Type: Journal Article
• Authors: Yousuf, Sameena; Enoch, Israel V. Muthu Vijayan
• Other Names: Takegami Shigehiko Academic Editor.
• Is Part Of: International journal of spectroscopy. Volume 2014(2014)
• Publication Date: 2014-06-05
• Subjects: Spectrum analysis -- Periodicals; Spectrum Analysis; Spectrum analysis; Periodicals; Periodicals; 543.5
• DOI: 10.1155/2014/562160
• Languages: English
• ISSNs: 1687-9449

Abstract

Abstract : Naringin-Bi (III) complex (Narb) was prepared and analysed by UV-Visible absorption and fluorescence measurements. The inclusion complex of Narb with β -Cyclodextrin ( β -CD) was characterized by the UV-Visible absorption, Infrared, scanning dlectron microscopic, and X-ray diffractometric techniques. The stoichiometry of the inclusion complex of Narb with β -CD was 1 : 1 with a binding constant of 5.18 × 10 2 mol −1 dm 3 . The interaction of Narb with Calf Thymus DNA (ctDNA) was investigated in the presence and the absence of β -CD. The binding constants for the interaction of Narb with ctDNA in the absence and the presence of β -CD were 1.29 × 10 5 mol −1 dm 3 and 6.89 × 10 4 mol −1 dm 3, respectively. The Stern-Volmer constants for the interaction of Narb with ctDNA in the absence and the presence of β -CD were 1.25 × 10 4 mol −1 dm 3 and 5.10 × 10 3 mol −1 dm 3, respectively. The lowering of the binding affinity and the K sv were observed for the interaction of Narb with ctDNA in the presence of β -CD.