Enhancement of Fluorescent Labeling via a Composited Thin Film. (3rd March 2014)

• Record Type: Journal Article
• Authors: Suyama, Taikei; Ji, Xiaowei; Zhang, Yaoju
• Other Names: Sahoo Nanda Gopal Academic Editor.
• Is Part Of: International journal of polymer science. Volume 2014(2014)
• Publication Date: 2014-03-03
• Subjects: Polymers -- Periodicals; Polymers; Periodicals; 547.7
• DOI: 10.1155/2014/921489
• Languages: English
• ISSNs: 1687-9422

Abstract

Abstract : Fluorescent labeling is the prevailing imaging technique in cell biological research. When statistical investigations on a large number of cells are involved, experimental study is required for both low magnification to get a reliable statistical population and high contrast to achieve accurate diagnosis on the nature of the cells' perturbation. As microscope objectives of low magnification generally yield low collection efficiency, such studies are limited by the fluorescence signal weakness. To overcome this technological insufficiency, Le Moal et al. proposed a method based on metal-coated substrates that enhanced the fluorescence process and improved collection efficiency in fluorescence microscope observation and that could be directly used with a common microscope setup. In this paper, we use an Ag-Si3 N4 -Ag multilayer film coated on the substrate and numerically analyse the optical behavior of a fluorophore which was placed above the composited film coated on the substrate. The results shows that by using an Ag-Si3 N4 -Ag composited film the fluorescence imaging can be enhanced remarkably.