Janus kinase/signal transducer and activator of transcription signaling pathway-related genes STAT3, SOCS3 and their role in thiram induced tibial dyschondroplasia chickens. (May 2021)

• Record Type: Journal Article
• Authors: Chen, Shu-ming; Jahejo, Ali Raza; Nabi, Fazul; Ahmed, Shakeel; Zhao, Jin-feng; Yu, Jin; Zhang, Chen-liang; Ning, Guan-bao; Zhang, Ding; Raza, Sayed Haidar Abbas; Tian, Wen-xia
• Is Part Of: Research in veterinary science. Volume 136(2021)
• Page Start: 25
• Page End: 31
• Publication Date: 2021-05
• Subjects: Chondrocytes -- Chicken -- Growth plate -- JAK/STAT -- p-STAT3 -- SOCS3; Veterinary medicine -- Periodicals; Veterinary Medicine -- Periodicals; Médecine vétérinaire -- Périodiques; Médecine vétérinaire -- Recherche -- Périodiques; Diergeneeskunde; 636.089
• DOI: 10.1016/j.rvsc.2021.01.024
• Languages: English
• ISSNs: 0034-5288

Abstract

Abstract: Pathogenicity of tibial dyschondroplasia (TD) in broiler chickens is not detected yet. Janus Kinase/Signal Transducer and Activator of Transcription (JAK-STAT) signaling pathway-related genes were investigated in thiram induced TD chickens. Real-time qPCR and immunohistochemical (IHC) technique were used to observe the expression changes of STAT3 and SOSC3 gene on days 1, 2, 4, 6 after feeding 100 mg·kg −1 thiram. Morphological, pathological, and histological results of this study suggested that chondrocyte cells were observed more damaged on day 6 than day 1, 2, and 4. Therefore, Lameness and damaged chondrocytes gradually increased from day 1 to 6. The mRNA expression level of STAT3 was observed insignificant ( P > 0.05) in thiram induced TD chickens' group of day 1. However, on days 2, 4, and 6, the expression was significant ( P < 0.05). SOCS3 increased in thiram group on days 1, 2 and 6, decreased on day 4 ( P < 0.05). The p-STAT3 and SOCS3 protein's protein localization was evaluated in the control and thiram-induced TD broiler chickens through IHC, suggesting that SOSC3 protein was observed significantly higher on days 1, 2, and 6 and down-regulated on day 4. p-STAT3 protein on thiram induced group was observed significantly upregulated on days 4 and 6. In conclusion, the differential expression of STAT3 and SOCS3 showed that the JAK-STAT signaling pathway might play an important role in regulating an abnormal proliferation, differentiation, or apoptosis of chondrocytes in TD at an early stage. Graphical abstract: JAK-STAT signaling pathway and related-genes (STAT3 and SOSC3) have an important role in chondrocytes proliferation and apoptosis. Unlabelled Image Highlights: JAK-STAT signaling pathway investigated in thiram induced TD chickens. The expression level of STAT3 and SOCS3 genes initially increased and then decreased. p-STAT3 and SOCS3 were expressed in PZ, PH, and HZ of the growth plate. p-STAT3 and SOCS3 showed that the JAK-STAT signaling pathway might play an important role in TD. Thiram may regulate the proliferation, apoptosis of chondrocytes through the JAK-STAT signaling pathway.