In Vitro Cytotoxicity Evaluation of Four Vital Pulp Therapy Materials on L929 Fibroblasts. (3rd March 2014)

• Record Type: Journal Article
• Authors: Wadajkar, Aniket S.; Ahn, Chul; Nguyen, Kytai T.; Zhu, Qiang; Komabayashi, Takashi
• Other Names: Merchant A. Academic Editor.; Pereira P. Academic Editor.
• Is Part Of: ISRN dentistry. Volume 2014(2014)
• Publication Date: 2014-03-03
• Subjects: Dentistry -- Periodicals; 617.6
• DOI: 10.1155/2014/191068
• Languages: English
• ISSNs: 2090-4371

Abstract

Abstract : The aim of this study was to evaluate cytotoxicity of direct pulp capping materials such as Dycal, Life, ProRoot MTA, and Super-Bond C&B on L929 fibroblasts. Freshly mixed or set materials were prepared and eluted by incubation with cell culture medium for working time period (fresh) or for 6 hours (set). The cells were exposed to media containing elutes for 24 hours, after which the cell survival was evaluated by MTS assays. In freshly mixed materials, average ± standard deviation % cell viabilities were 40.2 ± 14.0%, 43.7 ± 16.0%, 72.9 ± 12.7%, and 66.0 ± 13.6% for Dycal, Life, ProRoot MTA, and Super-Bond C&B, respectively. There was no statistical difference in cell viabilities among material groups, whereas in set materials, the cell viabilities were 48.7 ± 14.8%, 37.2 ± 10.6%, 46.7 ± 15.2%, and 100 ± 21.9% for Dycal, Life, ProRoot MTA, and Super-Bond C&B, respectively. Super-Bond C&B showed more cell viabilities than the other three material groups P < 0.05 . The four vital pulp therapy materials had similar cytotoxicity when the materials were fresh. Super-Bond C&B was less cytotoxic than Dycal, Life, and ProRoot MTA after the materials were set, which suggests the use of SB-C&B in future in vivo clinical investigations.