Anthocyanin Incorporated Dental Copolymer: Bacterial Growth Inhibition, Mechanical Properties, and Compound Release Rates and Stability by 1H NMR. (17th February 2014)

• Record Type: Journal Article
• Authors: Hrynash, Halyna; Pilly, Vinay Kumar; Mankovskaia, Alexandra; Xiong, Yaoyang; Nogueira Filho, Getulio; Bresciani, Eduardo; Lévesque, Céline Marie; Prakki, Anuradha
• Other Names: Abdalla Ali I. Academic Editor.
• Is Part Of: International journal of dentistry. Volume 2014(2014)
• Publication Date: 2014-02-17
• Subjects: Dentistry -- Periodicals; Dentistry; Dentistry -- Periodicals; Electronic journals; Periodicals; 617.6
• DOI: 10.1155/2014/289401
• Languages: English
• ISSNs: 1687-8728

Abstract

Abstract : Objective. To evaluate bacterial growth inhibition, mechanical properties, and compound release rate and stability of copolymers incorporated with anthocyanin (ACY; Vaccinium macrocarpon ). Methods. Resin samples were prepared (Bis-GMA/TEGDMA at 70/30 mol%) and incorporated with 2 w/w% of either ACY or chlorhexidine (CHX), except for the control group. Samples were individually immersed in a bacterial culture ( Streptococcus mutans ) for 24 h. Cell viability (n = 3 ) was assessed by counting the number of colony forming units on replica agar plates. Flexural strength (FS) and elastic modulus ( E ) were tested on a universal testing machine (n = 8 ). Compound release and chemical stability were evaluated by UV spectrophotometry and 1 H NMR (n = 3 ). Data were analyzed by one-way ANOVA and Tukey's test ( α = 0.05). Results. Both compounds inhibited S. mutans growth, with CHX being most effective (P < 0.05 ). Control resin had the lowest FS and E values, followed by ACY and CHX, with statistical difference between control and CHX groups for both mechanical properties (P < 0.05 ). The 24 h compound release rates were ACY: 1.33 μ g/mL and CHX: 1.92 μ g/mL. 1 H NMR spectra suggests that both compounds remained stable after being released in water. Conclusion. The present findings indicate that anthocyanins might be used as a natural antibacterial agent in resin based materials.