Comparison of 111In Leakage from Labeled Endocardial and Epicardial Cells: Impact on Modeling Viability of Cells to Be Transplanted into Myocardium. (26th April 2011)

• Record Type: Journal Article
• Authors: Blackwood, Kimberley J.; Sykes, Jane; Deans, Lela; Wisenberg, Gerald; Prato, Frank S.
• Other Names: Jaffer Farouc Academic Editor.
• Is Part Of: International journal of molecular imaging. Volume 2011(2011)
• Publication Date: 2011-04-26
• Subjects: Molecular diagnosis -- Periodicals; Molecular probes -- Diagnostic use -- Periodicals; Diagnostic imaging -- Periodicals; Molecular Imaging; Diagnostic imaging; Molecular diagnosis; Electronic journals; Periodicals; Periodicals; 616.0757
• DOI: 10.1155/2011/472375
• Languages: English
• ISSNs: 2090-1712

Abstract

Abstract : Introduction . Previously we proposed a cellular imaging technique to determine the surviving fraction of transplanted cells in vivo. Epicardial kinetics using Indium-111 determined the Debris Impulse Response Function (DIRF) and leakage coefficient parameters. Convolution-based modeling which corrected for these signal contributions indicated that 111 In activity was quantitative of cell viability with half-lives within 20 hrs to 37 days. We determine if the 37-day upper limit remains valid for endocardial injections by comparing previous epicardial cell leakage parameter estimates to those for endocardial cells. Methods . Normal canine myocardium was injected ( 111 In-tropolone) epicardially (9 injections) or endocardially (10 injections). Continuous whole body and SPECT scans for 5 hours were acquired with three weekly follow-up imaging sessions up to 20–26 days. Time-activity curves evaluated each injection type. Results . The epicardial and endocardial kinetics were not significantly different (Epi: 1286 ± 253 ; Endo: 1567 ± 470 hours P = .62 ). Conclusion . The original epicardial estimate of leakage kinetics has been validated for use in endocardial injections.