Identification and expression analysis of MicroRNAs in chicken spleen in a corticosterone-induced stress model. (May 2021)

• Record Type: Journal Article
• Authors: Su, Aru; Zhou, Yanting; Guo, Yujie; Yang, Xiuling; Zhang, Yanhua; Li, Wenting; Tian, Yadong; Li, Kui; Sun, Guirong; Jiang, Ruirui; Han, Ruili; Kang, Xiangtao; Yan, Fengbin
• Is Part Of: Research in veterinary science. Volume 136(2021)
• Page Start: 287
• Page End: 296
• Publication Date: 2021-05
• Subjects: Stress -- Corticosterone -- Immune function -- miRNA -- Chicken spleen; CORT Corticosterone -- GO Gene ontology -- KEGG kyoto encyclopedia of genes and genomes -- C_S CORT-treated spleen group -- B_S Control spleen group -- SIKE1 suppressor of IKBKE 1 -- CX3CL1 C-X3-C motif chemokine ligand 1 -- IL11Ra interleukin 11 receptor subunit alpha -- PIGR polymeric immunoglobulin receptor -- CDKN1A cyclin dependent kinase inhibitor 1A -- ADIPOQ adiponectin, C1Q and collagen domain containing -- GTSE1 G2 and S-phase expressed 1 -- ATP6V0A1 ATPase H+ transporting V0 subunit a1 -- ACE angiotensin I converting enzyme -- ATP6V0E2 ATPase H+ transporting V0 subunit e2; Veterinary medicine -- Periodicals; Veterinary Medicine -- Periodicals; Médecine vétérinaire -- Périodiques; Médecine vétérinaire -- Recherche -- Périodiques; Diergeneeskunde; 636.089
• DOI: 10.1016/j.rvsc.2021.02.023
• Languages: English
• ISSNs: 0034-5288

Abstract

Abstract: For investigating the effects of stress on the immune response of chickens, we established a corticosterone (CORT)-induced stress model by exogenous intake of CORT. Control group was fed with a basal diet and the stress model group was fed with a 30 mg/Kg CORT-treated diet in ad libitum conditions for 7 days. Then, we used RNA-seq technology to identify the expression pattern of miRNAs, target genes, and relevant pathways in chicken spleen. Results showed that 71 differentially expressed miRNAs (DEMs) were determined, 9 of which were significantly differentially expressed miRNAs (SDEMs), and 241 target genes of DEMs were predicted. GO annotation and KEGG pathway analysis were carried out to understand the role of the DEMs. Out of 287 significantly enriched GO terms, 37 were stress- or immune-related, such as response to light stimulus, detection of oxidative stress, and immune response in mucosal-associated lymphoid tissue. Out of 85 KEGG pathways, 8 were related to stress or immunity, such as cytokine-cytokine receptor interaction, JAK-STAT signaling pathway, and RLR signaling pathway. We then constructed the interaction networks between target genes from immune-related pathways and their DEMs. The analysis results suggested that some DEMs (gga-miR-17 family, gga-miR-15/16 family, gga-miR-2954 and gga-miR-34b-5p) and target genes ( SIKE1, CX3CL1, IL11Ra, PIGR, and CDKN1A ) were core miRNAs and genes. This study revealed the dynamic miRNA transcriptome, target genes and related pathways in chicken spleen under CORT-induced stress model, which provided a basis for studying the molecular mechanism of stress affecting immune function. Highlights: A corticosterone-induced stress model was constructed in Gushi chicken.. 71 differentially expressed miRNAs (DEMs) were identified by RNA-seq technology. 37 GO terms and 8 enrichment pathways of target genes were involved in stress reaction or immunity regulation. MiR-15/16 and miR-17 family and SIKE1, CX3CL1, IL11Ra were core miRNAs/mRNAs in stress-induced immunoregulation.