Design of synthetic microenvironments to promote the maturation of human pluripotent stem cell derived cardiomyocytes. Issue 7 (24th November 2020)

• Record Type: Journal Article
• Authors: Zhang, Rui; Guo, Tianwei; Han, Yu; Huang, Hongxin; Shi, Jiamin; Hu, Jiaxuan; Li, Hongjiao; Wang, Jianlin; Saleem, Amina; Zhou, Ping; Lan, Feng
• Is Part Of: Journal of biomedical materials research. Volume 109:Issue 7(2021)
• Page Start: 949
• Page End: 960
• Publication Date: 2020-11-24
• Subjects: cardiomyocyte -- human pluripotent stem cells -- maturation -- synthetic microenvironments; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/jbm.b.34759
• Languages: English
• ISSNs: 1552-4973

Abstract

Abstract: Cardiomyocyte like cells derived from human pluripotent stem cells (hPSC‐CMs) have a good application perspective in many fields such as disease modeling, drug screening and clinical treatment. However, these are severely hampered by the fact that hPSC‐CMs are immature compared to adult human cardiomyocytes. Therefore, many approaches such as genetic manipulation, biochemical factors supplement, mechanical stress, electrical stimulation and three‐dimensional culture have been developed to promote the maturation of hPSC‐CMs. Recently, establishing in vitro synthetic artificial microenvironments based on the in vivo development program of cardiomyocytes has achieved much attention due to their inherent properties such as stiffness, plasticity, nanotopography and chemical functionality. In this review, the achievements and deficiency of reported synthetic microenvironments that mainly discussed comprehensive biological, chemical, and physical factors, as well as three‐dimensional culture were mainly discussed, which have significance to improve the microenvironment design and accelerate the maturation of hPSC‐CMs.