Calycosin Alleviates Injury in Airway Epithelial Cells Caused by PM 2.5 Exposure via Activation of AMPK Signalling. (4th May 2021)

• Record Type: Journal Article
• Authors: Wang, Chunyan; Luo, Jingjing; Bai, Xiaoxue; Hua, Shucheng; Jie, Jing; Liu, Han; Gao, Jinying; Song, Lei
• Other Names: Hu Weicheng Academic Editor.
• Is Part Of: Evidence-based complementary and alternative medicine. Volume 2021(2021)
• Publication Date: 2021-05-04
• Subjects: Alternative medicine -- Periodicals; 615.505
• DOI: 10.1155/2021/8885716
• Languages: English
• ISSNs: 1741-427X

Abstract

Abstract : Introdution . Calycosin, a major active component in Astragali radix, has antitumour and anti-inflammation properties, but its effects on PM 2.5-induced injury in vitro and in vivo have not been clarified. Methods . Phospho-AMP-activated protein kinase (p-AMPK) and AMP-activated protein kinase (AMPK) were detected by western blot. Immunofluorescence staining was used to validate changes in the levels of nuclear factor kappa B (NF- к B) p65 nuclear translocation. Mice were administered intraperitoneally with calycosin one hour before anaesthesia and endotracheal instillation of PM 2.5. The extent of lung injury was evaluated in the H&E-stained lung sections. Apoptotic cells were detected by TUNEL staining. Results . Administration of calycosin was increased in PM 2.5-treated B2B cells in a dose-dependent manner in vitro . Fluorescence signals from anti-NF- к B p65 were increased in nuclei of cells pretreated with calycosin. The level of p-AMPK was increased by calycosin in vitro and in vivo . After pretreatment with compound C, the inhibitory effects of calycosin on cytotoxicity, levels of inflammatory cytokines and p-AMPK, and levels of NF- к B p65 nuclear translocation were not significantly decreased in vitro or in vivo . Conclusions . Calycosin effectively decreased the release of inflammatory cytokines and alleviated injury caused by PM 2.5. These effects were mediated through activation of AMPK to suppress NF- κ B signalling.