Polarography as a technique of choice for the evaluation of total antioxidant activity: The case study of selected Coprinus Comatus extracts and quinic acid, their antidiabetic ingredient. Issue 10 (19th May 2021)

• Record Type: Journal Article
• Authors: Karaman, Maja; Tesanovic, Kristina; Gorjanovic, Stanislava; Pastor, Ferenc T.; Simonovic, Mladen; Glumac, Miodrag; Pejin, Boris
• Is Part Of: Natural product research. Volume 35:Issue 10(2021)
• Page Start: 1711
• Page End: 1716
• Publication Date: 2021-05-19
• Subjects: cyclic polyols -- quinic acid -- antioxidativity -- polarography -- HPMC assay; Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2019.1628753
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract: This study was focused on in vitro screening of the total antioxidant activity of the selected extracts of the mushroom Coprinus comatus and quinic acid, one of their antidiabetic ingredients, by an uncommon electrochemical assay. Indeed, direct current (DC) polarographic HydroxoPerhydroxo Mercury(II) Complex (HPMC) assay based on decrease of anodic limiting current originating from HPMC formation in alkaline solutions of hydrogen peroxide at potential of mercury dissolution, observed upon gradual addition of antioxidants, was applied herein for the estimation of the natural products' antioxidativity. Quinic acid was found to exhibit most promising antioxidant potential (4.0 ± 0.2%µL −1 ) being ≈ 2-fold more active than the screened C. comatus extract samples. Actually, such a finding puts some light on the antioxidativity of cyclic polyols, well understimated class of organic compounds, compared to aromatic (poly)phenolics. As a low cost, easy-to handle and accurate this polarographic assay may be thoroughly recommended for much broader use.