Fabrication and evaluation of polycaprolactone/olive oil scaffolds by phase inversion for tissue engineering. (5th May 2021)

• Record Type: Journal Article
• Authors: Seyed Hakim, Ramina; Maghsoud, Zahra; Halabian, Raheleh
• Is Part Of: European polymer journal. Volume 150(2021)
• Publication Date: 2021-05-05
• Subjects: Polycaprolactone scaffold -- Phase inversion -- Olive oil -- Morphology control -- Antibacterial activity; Polymers -- Periodicals; Polymerization -- Periodicals; Polymères -- Périodiques; Polymérisation -- Périodiques; Polymerization; Polymers; Periodicals; Electronic journals; 547.705
• DOI: 10.1016/j.eurpolymj.2021.110394
• Languages: English
• ISSNs: 0014-3057

Abstract

Graphical abstract: Highlights: PCL/extra virgin olive oil (EVOO) scaffolds were fabricated by phase inversion method. The scaffold morphology was controlled merely by changing mixed solvent composition as well as EVOO content. Fabricated PCL/EVOO scaffolds may be appropriate for use as wound dressing considering their high antibacterial activity. Abstract: In this study, scaffolds containing polycaprolactone (PCL)/extra virgin olive oil (EVOO) at 1, 3, 5 and 7 wt% were fabricated using the phase inversion method. A combination of dimethylformamide (DMF) as the solvent and tetrahydrofuran (THF) as the co-solvent with different ratios was used to control the scaffold morphology. FTIR spectroscopy was applied for identifying the interaction between EVOO and PCL chains as well as the effect of mixed solvent composition on EVOO content of the scaffolds. The prepared scaffolds had a sponge-like structure where their average pore size was reduced by increasing the percentage of THF or decreasing the amount of EVOO in the casting solution. The measurement of water contact angle (WCA) showed that the oil increment in the casting solution had a direct effect on surface hydrophobicity. Evaluation of the mechanical properties of the scaffolds implied that the scaffold containing 3 wt% EVOO had the best mechanical properties. The results of the MTT assay analysis indicated that the scaffolds were non-toxic and suitable for cell growth. Furthermore, the high antibacterial activity of the scaffolds against Gram-negative Pseudomonas aeruginosa bacteria and Gram-positive Staphylococcus aureus was confirmed.