Production and characterization of a mouse monoclonal antibody against smallmouth bass (Micropterus dolomieu) IgM. Issue 113 (June 2021)

• Record Type: Journal Article
• Authors: Ottinger, Christopher A.; Smith, Cheyenne R.; Blazer, Vicki S.; Iwanowicz, Luke R.; Vogelbein, Mary Ann; Kaattari, Stephen
• Is Part Of: Fish & shellfish immunology. Issue 113(2021)
• Page Start: 20
• Page End: 23
• Publication Date: 2021-06
• Subjects: Smallmouth bass -- Micropterus dolomieu -- Largemouth bass -- Micropterus salmoides -- Bluegill -- Lepomis macrochirus -- Monoclonal antibody -- Total plasma immunoglobulin -- ELISA; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2021.03.006
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: A murine monoclonal antibody (mAb, IgG2a) was produced for the detection of smallmouth bass ( Micropterus dolomieu ) immunoglobulin (IgM). The antibody is specific for IgM heavy chain and was shown to also recognize the Ig heavy chain of largemouth bass ( Micropterus salmoides ) and bluegill ( Lepomis macrochirus ) using Western Blot analysis of plasma from 9 teleost taxa. When applied to the analysis of smallmouth bass total plasma IgM using ELISA, the mAb was found to be effective when used in an inhibition kinetic assay. Highlights: Mouse IgG2a Monoclonal antibody produced against smallmouth bass IgM. Antibody reacts with IgM from smallmouth and largemouth bass as well as bluegill. Application of antibody in Western Blot and ELISA demonstrated.