Dietary cinnamaldehyde nanoemulsion boosts growth and transcriptomes of antioxidant and immune related genes to fight Streptococcus agalactiae infection in Nile tilapia (Oreochromis niloticus). Issue 113 (June 2021)

• Record Type: Journal Article
• Authors: Abd El-Hamid, Marwa I.; Ibrahim, Seham M.; Eldemery, Fatma; El-Mandrawy, Shefaa A.M.; Metwally, Aya Sh.; Khalifa, Eman; Elnahriry, Shimaa S.; Ibrahim, Doaa
• Is Part Of: Fish & shellfish immunology. Issue 113(2021)
• Page Start: 96
• Page End: 105
• Publication Date: 2021-06
• Subjects: Cinnamaldehyde nanoemulsion -- Nile tilapia -- Growth performance -- Antioxidant enzymes -- Cytokines -- S. agalactiae; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2021.03.021
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: The present study was conducted to investigate the effects of dietary cinnamaldehyde nanoemulsion (CNE) on growth, digestive activities, antioxidant and immune responses and resistance against Stre ptococcus agalactiae ( S. agalactiae ) in Nile tilapia. Four experimental diets were formulated containing CNE at levels of 0, 100, 200 and 300 mg/kg diet for 12 weeks. At the end of the experiment, all fish were challenged by S . agalactiae. The results showed that the final body weight was increased in fish groups fed 200 and 300 mg CNE/kg diet by 18.4 and 17.2% with respect to the control group. Moreover, feed conversion ratio and digestive enzymes' activities were improved in groups fed 200 and 300 then 100 mg of dietary CNE/kg diet. Groups fed CNE exhibited a significant increase in serum immune-related parameters when compared with control group. Additionally, the hypocholesterolemic effects was achieved after CNE feeding unlike the control group in a dose dependent manner. With increasing dietary CNE levels, genes expression of cytokines and antioxidant enzymes were upregulated. Less severe adverse clinical symptoms and respectable cumulative mortalities associated with S. agalactiae infection were observed in fish fed CNE. To our knowledge, this study was the first offering a protective effect of CNE against S. agalactiae infection in Nile tilapia with a maximum down-regulation of cylE and hylB virulence genes expression noticed in group fed 300 mg of CNE/kg diet (up to 0.10 and 0.19- fold, respectively). Therefore, the present study recommended that an incorporation of CNE at level of 300 mg/kg diet for Nile tilapia could promote their growth, enhance their immunity and antioxidant status and provide protection against virulent S. agalactiae. Highlights: Growth performance of Nile tilapia can be boosted by dietary CNE. CNE supplemented diets potentiated the expression of SOD, CAT, GPx genes in Nile tilapia. Dietary CNE can modulate immune responses of Nile tilapia. Dietary CNE could reinforce resistance against Streptococcus agalactiae infection in Nile tilapia.