The performance of 3D bioscaffolding based on a human periodontal ligament stem cell printing technique. Issue 7 (16th October 2020)

• Record Type: Journal Article
• Authors: Tian, Yinping; Liu, Minyi; Liu, Yaoyao; Shi, Changzheng; Wang, Yayu; Liu, Tong; Huang, Yi; Zhong, Peihua; Dai, Jian; Liu, Xiangning
• Is Part Of: Journal of biomedical materials research. Volume 109:Issue 7(2021)
• Page Start: 1209
• Page End: 1219
• Publication Date: 2020-10-16
• Subjects: 3D bioprinting -- bioink -- cell bioscaffolds -- human periodontal ligament stem cells -- hydrogels; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/jbm.a.37114
• Languages: English
• ISSNs: 1549-3296

Abstract

Abstract: Bone tissue plays an important role in supporting and protecting the structure and function of the human body. Bone defects are a common source of injury and there are many reconstruction challenges in clinical practice. However, 3D bioprinting of scaffolds provides a promising solution. Hydrogels have emerged as biomaterials with good biocompatibility and are now widely used as cell‐loaded materials for bioprinting. This study involved three steps: First, sodium alginate (SA), gelatin (Gel), and nano‐hydroxyapatite (na‐HA) were mixed into a hydrogel and its rheological properties assessed to identify the optimum slurry for printing. Second, SA/Gel/na‐HA bioscaffolds were printed using 3D bioprinting technology and their physical properties characterized for surface morphology, swelling, and mechanical properties. Finally, human periodontal ligament stem cells (hPDLSCs) were mixed with SA/Gel/na‐HA printing slurry to create a "bioink" to prepare SA/Gel/na‐HA/ hPDLSCs cell bioscaffolds. These were tested for biocompatibility and osteogenic differentiation performance using live/dead cell staining, cell adhesion, cell proliferation, and alkaline phosphatase activity. The SA/Gel/na‐HA hydrogel exhibited shear‐thinning behavior. The equilibrium swelling of the bioscaffold was 125.9%, the compression stress was 0.671 MPa, and the compression elastic modulus was 8.27 MPa. The SA/Gel/na‐HA/hPDLSCs cell bioscaffolds caused effective stimulation of cell survival, proliferation, and osteoblast differentiation. Therefore, the SA/Gel/na‐HA/hPDLSCs cell bioscaffolds displayed potential as a material for bone defect reconstruction.