Ficolin‐3 in chronic Chagas disease: Low serum levels associated with the risk of cardiac insufficiency. (18th March 2021)

• Record Type: Journal Article
• Authors: Lidani, Kárita Cláudia Freitas; Andrade, Fabiana Antunes; Beltrame, Marcia Holsbach; Chakravarti, Indira; Tizzot, Maria Regina; Cavalcanti, Edneia Oliveira; Sandri, Thaisa Lucas; Luz, Paola Rosa; Messias‐Reason, Iara J.
• Is Part Of: Parasite immunology. Volume 43:Number 6(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-03-18
• Subjects: Chagas disease -- complement -- ficolin‐3 -- inflammation -- Trypanosoma spp; Veterinary parasitology -- Immunological aspects -- Periodicals; Host-parasite relationships -- Immunological aspects -- Periodicals; 571.96
• DOI: 10.1111/pim.12829
• Languages: English
• ISSNs: 0141-9838

Abstract

Abstract: Aims: To investigate whether FCN3 polymorphisms and circulating ficolin‐3 levels were associated with clinical forms of chronic Chagas disease (CD) and to assess their potential use as biomarkers for the disease or its severity. Methods and Results: FCN3 polymorphisms ( g.1637delC (rs532781899) in exon 5; g.3524_3532insTATTTGGCC (rs28362807) in intron 5 and g.4473C > A ) (rs4494157) in intron 7) were determined in 178 chronic CD patients (65 asymptomatic, 68 cardiac, 21 digestive and 24 cardiodigestive), and 285 healthy controls by sequence‐specific PCR. Ficolin‐3 serum levels, measured by ELISA in 80 patients and 80 controls, did not differ between groups. On the other hand, ficolin‐3 levels were positively correlated with left ventricular ejection fraction ( P = .002; r = .5), with lower levels associated with increased risk of cardiac insufficiency ( P = .033; OR 7.21, 95%IC 1.17‐44.4). Ficolin‐3 levels were positively correlated with ficolin‐2 ( P = .021; r = .63), and negatively with MBL ( P = .002; r = −.36) and pentraxin‐3 ( P = .04; r = −.32) levels. No significant results were observed for the investigated FCN3 polymorphisms and CD. The g.1637del/1637C heterozygotes presented lower ficolin‐3 levels than g.1637C/1637C homozygotes in the control group ( P = .023). Conclusion: Low ficolin‐3 levels may play a role in the pathophysiology of cardiac insufficiency associated with CD.