Identification of Potential Biomarkers in Peripheral Blood Supernatants of South African Patients with Syphilitic and Herpetic Uveitis. (17th February 2021)

• Record Type: Journal Article
• Authors: Andreae, Corinna D.; Smit, Derrick P.; Makhoba, Nonjabulo S.; Kidd, Martin; Walzl, Gerhard; Chegou, Novel N.
• Is Part Of: Ocular immunology and inflammation. Volume 29:Number 2(2021)
• Page Start: 299
• Page End: 307
• Publication Date: 2021-02-17
• Subjects: Biomarkers -- herpes -- South Africa -- syphilis -- Uveitis; Eye -- Diseases -- Immunological aspects -- Periodicals; Eye -- Inflammation -- Periodicals; 617.7
• DOI: 10.1080/09273948.2019.1674341
• Languages: English
• ISSNs: 0927-3948

Abstract

ABSTRACT: Purpose : To identify potential diagnostic biomarkers for herpetic and syphilitic uveitis. Methods : Blood samples were collected from 92 uveitis patients. Concentrations of 47 biomarkers were evaluated in unstimulated Quantiferon supernatants using the Luminex platform. Results : Results showed 11 patients (12%) had herpetic uveitis, 11 (12%) syphilis, 40 (43.5%) other infectious causes, 16 (17.4%) established noninfectious causes and 14 (15.2%) were idiopathic. Biomarker analysis revealed three proteins (Apo-A1, Apo-CIII, CRP) that differed between syphilis and other causes. A three-marker biosignature (CCL4/MIP-1β, Apo-CIII and CRP) separated syphilis from other groups with AUC = 0.83 (95% CI: 0.68–0.98). Apo-CIII and CRP differed between herpetic cases and other groups ( p < .05). A three-analyte biosignature (Apo-A1, SAP and CRP) separated the herpetic group from other groups with AUC = 0.79 (95% CI: 0.65–0.93). Conclusion : We have identified candidate biomarkers with potential to differentiate between herpetic, syphilitic and other causes of uveitis. These results warrant further investigation in larger future studies.