Multilayer fibroin/chitosan oligosaccharide lactate and pullulan immunomodulatory patch for treatment of hernia and prevention of intraperitoneal adhesion. (1st August 2021)

• Record Type: Journal Article
• Authors: Akbaba, S.; Atila, D.; Keskin, D.; Tezcaner, T.; Tezcaner, A.
• Is Part Of: Carbohydrate polymers. Volume 265(2021)
• Publication Date: 2021-08-01
• Subjects: Hernia -- Immunomodulation -- Peritoneal adhesion -- Regeneration; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2021.118066
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Pullulan hydrogel prevents cell adhesion in vitro . Fibroin/chitosan oligosaccharide lactate promotes cell viability and proliferation. Chitosan oligosaccharide lactate lowers nitric oxide release from RAW 264.7. Chitosan oligosaccharide lactate modulates NFκB expression of THP-1 monocytes. Abstract: This study aims to develop a novel intraperitoneal two- or three-layered patch with immunomodulatory property for treatment of hernia, regeneration of abdominal wall and prevention of intraperitoneal adhesions. Polypropylene (PP) mesh, middle layer, was intended to provide mechanical support whereas pullulan (PUL) hydrogel coating layer was designed to prevent intraperitoneal adhesions. Fibroin/chitosan oligosaccharide lactate (F/COS) layer electrospun on one side of pullulan was chosen for immunomodulation and abdominal wall regeneration. Physical and mechanical properties and regenerative capacity of intraperitoneal patches were determined. Immunomodulatory property of electrospun layer and whole patch was studied by determining nitric oxide amount produced by RAW 264.7 macrophages. 25 % (w/v) PUL hydrogel and F/COS with 90:10 (w/w) ratio yielded optimal results. Here, we report that fabricated intraperitoneal patches successfully prevented cell adhesion on one side and increased cell viability and proliferation on other side, along with immunomodulation, in vitro .