Following Electroenzymatic Hydrogen Production by Rotating Ring–Disk Electrochemistry and Mass Spectrometry. Issue 18 (22nd March 2021)

• Record Type: Journal Article
• Authors: Khushvakov, Jaloliddin; Nussbaum, Robin; Cadoux, Cécile; Duan, Jifu; Stripp, Sven T.; Milton, Ross D.
• Is Part Of: Angewandte Chemie international edition. Volume 60:Issue 18(2021)
• Page Start: 10001
• Page End: 10006
• Publication Date: 2021-03-22
• Subjects: [FeFe] -- enzymatic electrochemistry -- hydrogen -- kinetic isotope effect -- metalloenzymes; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.202100863
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: Gas‐processing metalloenzymes are of interest to future bio‐ and bioinspired technologies. Of particular importance are hydrogenases and nitrogenases, which both produce molecular hydrogen (H2 ) from proton (H + ) reduction. Herein, we report on the use of rotating ring–disk electrochemistry (RRDE) and mass spectrometry (MS) to follow the production of H2 and isotopes produced from deuteron (D + ) reduction (HD and D2 ) using the [FeFe]‐hydrogenase from Clostridium pasteurianum, a model hydrogen‐evolving metalloenzyme. This facilitates enzymology studies independent of non‐innocent chemical reductants. We anticipate that these approaches will be of value in resolving the catalytic mechanisms of H2 ‐producing metalloenzymes and the design of bioinspired catalysts for H2 production and N2 fixation. Abstract : Metalloenzymes are of interest for biotechnology and bioinspired catalyst design. The use of rotating ring–disk electrochemistry and on‐line mass spectrometry is reported for following electroenzymatic hydrogen formation and isotopic product distribution.