Primary human mesothelial cell culture in the evaluation of the inflammatory response to different sclerosing agents used for pleurodesis. Issue 8 (1st May 2021)

• Record Type: Journal Article
• Authors: Mierzejewski, Michal; Paplinska‐Goryca, Magdalena; Korczynski, Piotr; Krenke, Rafal
• Is Part Of: Physiological reports. Volume 9:Issue 8(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-05-01
• Subjects: iodopovidone -- mesothelial cells -- pleural effusion -- pleural fluid -- pleurodesis -- sclerosing agents -- talc; Physiology -- Periodicals; 571
• DOI: 10.14814/phy2.14846
• Languages: English
• ISSNs: 2051-817X

Abstract

Abstract: The mechanisms of chemical pleurodesis are still not fully explained. We aimed to evaluate the feasibility of using primary biopsy‐derived human mesothelial cells to establish an in vitro culture and to assess the response of pleural mesothelial cells to different sclerosing agents. Talc, povidone‐iodine, doxycycline, and TGF‐β were used at different doses to stimulate pleural mesothelial cells. After 6 and 24 h, mRNA expression of interleukin (IL)‐1β, IL‐6, IL‐8, TGF‐β, MCP‐1, IL‐17A, and MMP9 was measured in cultured cells, and the protein level of IL‐1β, IL‐6, and IL‐8 was measured in the culture supernatant. The most pronounced response was observed after talc exposure. It was expressed as an increase in IL‐1β concentration in culture supernatant after 24 h of higher talc dose stimulation compared to 6 h of stimulation (17.14 pg/ml [11.96–33.32 pg/ml] vs. 1.84 pg/ml [1.81–1.90 pg/ml], p = 0.02). We showed that culture pleural mesothelial cells isolated from pleura biopsy specimens is feasible. Inflammatory responses of mesothelial cells to different sclerosants were highly variable with no consistent pattern of mesothelium reaction neither in terms of different sclerosing agents nor in the time of the most significant reaction. We demonstrated that pro‐inflammatory mesothelial response includes an increase in IL‐1β mRNA expression and protein production. This may suggest the role of IL‐1β in the formation and maintenance of the inflammatory response during pleurodesis. Abstract : We performed the prospective study using primary, biopsy‐derived human mesothelial cells to establish an in vitro culture and to assess the response of pleural mesothelial cells to four different sclerosing agents (talc, povidone‐iodine, doxycycline, and transforming growth factor β) in different doses. Our study shows, that it is feasible to culture pleural mesothelial cells isolated from pleura biopsy specimens, that can be used for research on pleurodesis mechanisms. We found that pro‐inflammatory mesothelial response includes an increase in interleukin (IL)‐1β mRNA expression and protein production, which suggests the role of IL‐1β in the formation and maintenance of the inflammatory response during pleurodesis. ​