Efficiency of electron beam over gamma rays to induce desirable grain-type mutation in rice (Oryza sativa L.). (4th May 2021)

• Record Type: Journal Article
• Authors: Gowthami, R.; Vanniarajan, C.; Souframanien, J.; Veni, K.; Renganathan, V. G.
• Is Part Of: International journal of radiation biology. Volume 97:Number 5(2021)
• Page Start: 727
• Page End: 736
• Publication Date: 2021-05-04
• Subjects: Electron beam -- gamma rays -- rice -- grain-type mutants; Radiation -- Physiological effect -- Periodicals; Radiobiology -- Periodicals; 571.45
• DOI: 10.1080/09553002.2021.1889702
• Languages: English
• ISSNs: 0955-3002

Abstract

Abstract: Purpose: Rice is the predominant crop of Tamil Nadu state, India that occupies about 30% of the total cropped area. However, grain type and quality are the critical traits that determine the market value and domestic consumption rice variety. Most of the households of Tamil Nadu, India prefer to consume medium slender to fine grain type of rice. Hence, the present study was conducted to induce medium slender grain type in popular rice variety ADT 37 (Aduthurai 37), a short bold rice variety using gamma rays (GR) and electron beam (EB) mutagens. Materials and methods: Healthy, dried seeds (12.0% moisture content) of ADT 37 rice variety were exposed to various doses of GR (100–500 Gy) and EB (200–600 Gy). The irradiated population were maintained up to M4 generation by plant to progeny row basis to identify stable mutants for grain-type variation. The selected grain-type mutants (medium slender- and slender-type mutants) in M4 generation were characterized for phenotypic and grain quality traits. Results: A high frequency of desirable grain-type variation was observed in EB-irradiated population than gamma-irradiated population. A total of 25 grain-type mutants (long slender and medium slender) were obtained in M4 generation of ADT 37 variety. The morphological characterization and cooking quality assessment of the 'grain-type' mutants revealed that six out of 25 mutants viz., M-3 (Mutant-3), M-5, M-9, M-10, M-13 and M-15 recorded single plant yield of more than 30 g. There was non-significant variation in yield per plant (g) among the mutants and control (parent) due to key changes in grain type and thousand grain weight. Conclusion: EB showed higher mutation frequency, mutagenic effectiveness and efficiency than the GR in inducing both chlorophyll and viable mutants. This study revealed that the percentage contribution of the EB was 2.57 times higher than that of GR in obtaining desirable slender and medium slender grain-type mutants. The grain-type mutants obtained in the present study can be either directly released as variety or used as parents in hybridization program of rice crop improvement.