MiR-181a-5p inhibits goose granulosa cell viability by targeting SIRT1. Issue 3 (4th May 2021)

• Record Type: Journal Article
• Authors: Rong, Y.; Mo, Y.; Liu, Y.; Deng, Y.; Hu, S.; Li, L.; Hu, J.; Hu, B.; He, H.; Wang, J.
• Is Part Of: British poultry science. Volume 62:Issue 3(2021)
• Page Start: 373
• Page End: 378
• Publication Date: 2021-05-04
• Subjects: Granulosa cells -- miR-181a-5p -- SIRT1 -- cell viability -- geese; Poultry -- Periodicals; Poultry industry -- Periodicals; 636.500941
• DOI: 10.1080/00071668.2020.1870660
• Languages: English
• ISSNs: 0007-1668

Abstract

ABSTRACT: 1. Granulosa cells (GCs) are involved in folliculogenesis, follicular development, and atresia. Previous studies have shown that microRNA-181a-5p (miR-181a-5p) and sirtuin 1 ( SIRT1 ) are involved in GC proliferation and apoptosis, and SIRT1 has been predicted as one target of miR-181a-5p. However, there are few studies with poultry. 2. Quantitative real-time PCR (qRT-PCR) was used to detect the expression level of miR-181a-5p in granulosa layers during geese ovarian follicular development. A methyl thiazolyl tetrazolium (MTT) assay was performed to assess the viability of geese granulosa cells treated with miR-181a-5p mimic or inhibitor. The binding sites between the SIRT1 3′-UTR region and miR-181a-5p were evaluated using a luciferase reporter assay system. SIRT1 mRNA levels were detected using qRT-PCR after transfection with miR-181a-5p mimic and inhibitor. 3. The miR-181a-5p suppressed geese GC viability and regulated the mRNA expression of viability-related genes in geese GCs. SIRT1 was a target gene of miR-181a-5p and miR-181a-5p suppressed its mRNA expression. 4. The miR-181a-5p may target and inhibit SIRT1 expression, thus suppressing GC viability by regulating viability-related key genes.