A Comparative Estimation of Alprazolam in Pharmaceutical Formulations by Validated HPLC and HPTLC Techniques. Issue 2 (4th March 2021)

• Record Type: Journal Article
• Authors: Sharma, Aradhana; Gaurav, Kumar; Srivastava, Richa
• Is Part Of: Analytical chemistry letters. Volume 11:Issue 2(2021)
• Page Start: 187
• Page End: 197
• Publication Date: 2021-03-04
• Subjects: Alprazolam -- HPLC -- HPTLC -- Phosphate buffer -- validation -- analysis; Chemistry, Analytic -- Periodicals; 543.05
• DOI: 10.1080/22297928.2021.1892520
• Languages: English
• ISSNs: 2229-7928

Abstract

Abstract: The aim of present work is to develop and validate two simple and specific methods for the estimation of alprazolam hydrochloride (a psychoactive drug) in pharmaceutical formulations. The first method involves HPLC separation on the Cosmosil C-8 column using acetonitrile and potassium phosphate buffer (pH 6.0±0.1) (40:60 v/v) as mobile phase. The peak was obtained at 3.01±0.02 min. The effluent was monitored at 254 nm. The second method is based on HPTLC using Acetic acid: water: methanol: ethyl acetate (2:15:20:80 v/v/v/ v) as mobile phase and detection of the spot was carried out at 254 nm. The Rf value was found to be 0.76±0.02. The methods were validated as per ICH guidelines. The linearity curves were found to be linear over the concentration range 50-150 μg/ ml for HPLC and 50-400 ng/spot for HPTLC with a high correlation coefficient (R 2 = 0.991 and 0.9987 respectively). The limit of detection and limit of quantification were found to be 1.66 and 5.04 μg/ml for HPLC and14.84 and 44.097 μg/ ml for HPTLC, respectively. The proposed methods are successfully used for the quality control of alprazolam in commercial form. Statistical analysis results confirmed that the methods are selective and precise.