Production of β-glucanase and protease from Bacillus velezensis strain isolated from the manure of piglets. Issue 5 (3rd May 2021)

• Record Type: Journal Article
• Authors: Khalid, Anam; Ye, Miao; Wei, Chunjie; Dai, Binghong; Yang, Ru; Huang, Shoujun; Wang, Zaigui
• Is Part Of: Preparative biochemistry & biotechnology. Volume 51:Issue 5(2021)
• Page Start: 497
• Page End: 510
• Publication Date: 2021-05-03
• Subjects: Bacillus velezensis -- β-glucanase -- enzyme properties -- fermentation conditions -- protease; Biochemistry -- Technique -- Periodicals; Biotechnology -- Technique -- Periodicals; Biochemistry -- methods -- Periodicals; Biotechnology -- methods -- Periodicals; 660.6
• DOI: 10.1080/10826068.2020.1833344
• Languages: English
• ISSNs: 1082-6068

Abstract

Abstract: In this study, a strain producing β-glucanase and protease, identified as Bacillus velezensis Y1, was isolated from the manure of piglet. We attempted to produce β-glucanase and protease after optimization of various process parameters with the submerged fermentation. The effects of each factor on producing β-glucanase and protease were as follows: temperature > time > pH > loaded liquid volume. The properties of the β-glucanase showed that the most suitable reaction temperature was 65 °C and pH was 6.0. However for protease optimum reaction temperature was 50 °C, and pH was 6.0. The amplified PCR fragments of β-glucanase and protease were 1434 bp containing an open reading frame of 1413 bp encoding a protein with 444 amino acids and 1752 bp containing an open reading frame of 1521 bp encoding a protein with 506 amino acids, respectively. So, the study demonstrated a viable approach of using newly identified B. velezensis Y1 strain for the maximum yield of two industrially important enzymes. Graphical Abstract: