ELONGATED HYPOCOTYL 5 mediates blue light-induced starch degradation in tomato. (29th December 2020)

• Record Type: Journal Article
• Authors: Dong, Han; Hu, Chaoyi; Liu, Chaochao; Wang, Jiachun; Zhou, Yanhong; Yu, Jingquan
• Editors: Beckles, Diane
• Is Part Of: Journal of experimental botany. Volume 72:Number 7(2021)
• Page Start: 2627
• Page End: 2641
• Publication Date: 2020-12-29
• Subjects: Blue light -- cryptochrome -- HY5 -- Solanum lycopersicum -- starch degradation -- sugar accumulation -- tomato; Botany -- Periodicals; Botany, Experimental -- Periodicals; Plant physiology -- Periodicals; 580
• DOI: 10.1093/jxb/eraa604
• Languages: English
• ISSNs: 0022-0957

Abstract

Abstract : The blue light receptor CRY1a up-regulates transcript and protein levels of HY5 in tomato. HY5 directly binds to the promoters of several starch degradation-related genes to activate their transcription, inducing starch breakdown. Abstract: Starch is the major storage carbohydrate in plants, and its metabolism in chloroplasts depends mainly on light. However, the mechanism through which photoreceptors regulate starch metabolism in chloroplasts is unclear. In this study, we found that the cryptochrome 1a (CRY1a)-mediated blue light signal is critical for regulating starch accumulation by inducing starch degradation through the transcription factor HY5 in chloroplasts in tomato. cry1a mutants and HY5 -RNAi plants accumulated more starch and presented lower transcript levels of starch degradation-related genes in their leaves than wild-type plants. Blue light significantly induced the transcription of starch degradation-related genes in wild-type and CRY1a - or HY5 -overexpressing plants but had little effect in cry1a and HY5 -RNAi plants. Dual-luciferase assays, electrophoretic mobility shift assays, and chromatin immunoprecipitation–qPCR revealed that HY5 could activate the starch degradation-related genes PWD, BAM1, BAM3, BAM8, MEX1, and DPE1 by directly binding to their promoters. Silencing of HY5 and these starch degradation-related genes in CRY1a -overexpressing plants led to increased accumulation of starch and decreased accumulation of soluble sugars. The findings presented here not only deepen our understanding of how light controls starch degradation and sugar accumulation but also allow us to explore potential targets for improving crop quality.