Atrazine impairs testicular function in BalB/c mice by affecting Leydig cells. (15th May 2021)

• Record Type: Journal Article
• Authors: Abarikwu, Sunny O.; Costa, Guilherme M.J.; de Lima e Martins Lara, Nathalia; Lacerda, Samyra M.S.N.; de França, Luiz R.
• Is Part Of: Toxicology. Volume 455(2021)
• Publication Date: 2021-05-15
• Subjects: Testis -- Spermatogenesis -- Leydig cells -- 3β-HSD -- Testosterone -- Apoptosis -- Cell cycle; Toxicology -- Periodicals; Chemicals -- Physiological effect -- Periodicals; 615.9005
• DOI: 10.1016/j.tox.2021.152761
• Languages: English
• ISSNs: 0300-483X

Abstract

Graphical abstract: Highlights: Atrazine exposure decreased Leydig cell's 3β-HSD expression. Caspase-3 expression in Leydig cells was increased by atrazine. TUNEL staining confirms apoptotic Leydig cells death by atrazine. Testosterone level was decreased by atrazine. Leydig cell is the primary target for atrazine in the testis. Abstract: Several studies have reported the effects of atrazine on the gonads of many experimental models. However, the short-term effects of in vivo exposure to atrazine on the testes of mice are not well clarified. Here we reported that adult BalB/c mice exposed to atrazine (50 mg kg −1 body weight) by gavage for three consecutive days have reduced numbers of 3β-hydroxysteroid dehydrogenase positive Leydig cells (LCs), associated with increased in situ cell death fluorescence and caspase-3 immuno-expression in the testes. Consequently, immunostaining for cell cycle gene regulators showed increased expressions of p45, accompanied with increased expressions of cyclin D2 and E2. Histological observations of the gonads showed reduced number of germ cells in particular areas, sloughed seminiferous epithelium, presence of giant apoptotic cells close to the seminiferous tubule lumen and in the epididymal lumen along with low numbers of Leydig cells in the testicular interstitial areas. Similarly, LCs isolated from the testes of BalB/c mice that were exposed to atrazine (0.5, 25, 50 mg kg −1 body weight) in the same manner as in the first experiment presented dose-dependent increased caspase-3 activity, decreased cell viability, intratesticular and serum testosterone concentrations and LCs testosterone secretion. In summary, atrazine appears to directly decrease the number of testosterone secreting LCs in mice through apoptosis.