Identification of Anaplasma spp. in Tian Shan wapiti deer (Cervus elaphus songaricus) in Xinjiang, China. (April 2021)

• Record Type: Journal Article
• Authors: Li, Tao; Cui, Yanyan; Xiao, Jinxiu; Jiang, Yuxi; Ning, Changshen; Qi, Meng; Tao, Dayong
• Is Part Of: International journal for parasitology. Volume 14(2021)
• Page Start: 157
• Page End: 160
• Publication Date: 2021-04
• Subjects: Anaplasma -- Phylogenetic analysis -- Tian Shan wapiti -- PCR; Parasites -- Periodicals; Parasitology -- Periodicals; Animals -- Periodicals; Wildlife diseases -- Periodicals; Parasites -- Periodicals; Animals, Wild -- Periodicals; Animals; Parasites; Parasitology; Wildlife diseases; Periodicals; 591.7857
• DOI: 10.1016/j.ijppaw.2021.02.008
• Languages: English
• ISSNs: 2213-2244

Abstract

Abstract: Anaplasma spp. are important zoonotic tick-borne pathogens that impact on human health. There are few reports on the prevalence and molecular genetic characteristics of Cervidae species in China. The purpose of this study, therefore, was to investigate the presence of Anaplasma spp. in blood samples of Tian Shan wapiti ( Cervus elaphus songaricus ) in the Xinjiang Uygur Autonomous Region of China, and conduct phylogenetic analyses. A total of 50 blood samples (wild deer n = 26, and captive deer n = 24) were collected from the deer. PCR was used to detect Anaplasma spp. in the blood samples. Forty percent (20) of the samples were found to contain Anaplasma spp. Three Anaplasma species DNA were detected in deer blood samples: A. bovis (n = 13), A. ovis (n = 18), and A. phagocytophilum (n = 11). Among the 20 Anaplasma spp. positive samples, 14 were mixed infection of two or three pathogens. The prevalence of Anaplasma species in wild deer was significantly higher than that of captive deer, 73.1% (19) vs 4.2% (1) respectively, ( p < 0.01). Two A. ovis sequence types (AB1, and AB2), three A. ovis sequence types (AO1-AO3), and one A. phagocytophilum sequence type (AP1) were obtained in this study. The sequences of AO1 shared 100% identity with a human isolate from Cyprus. Our results suggest that wild deer are more likely to become infected with Anaplasma spp. than captive individuals, and thus, could potentially transmit pathogens to humans. Graphical abstract: Image 1 Highlights: This is the first report of Anaplasma spp. in Tian Shan wapiti in Xinjiang, China. Wild Tian Shan wapiti are more to infected with Anaplasma species. Tian Shan wapiti may pose as a reservoir for Anaplasma spp.