Neuroprotective effect of CuATSM in mice stroke model by ameliorating oxidative stress. (May 2021)

• Record Type: Journal Article
• Authors: Shi, Xiaowen; Ohta, Yasuyuki; Nakano, Yumiko; Liu, Xia; Tadokoro, Koh; Feng, Tian; Nomura, Emi; Tsunoda, Keiichiro; Sasaki, Ryo; Matsumoto, Namiko; Osakada, Yosuke; Bian, Yuting; Bian, Zhihong; Omote, Yoshio; Takemoto, Mami; Hishikawa, Nozomi; Yamashita, Toru; Abe, Koji
• Is Part Of: Neuroscience research. Volume 166(2021)
• Page Start: 55
• Page End: 61
• Publication Date: 2021-05
• Subjects: ABC avidin-biotin-peroxidase complex -- ARE antioxidant response element -- BSA bovine serum albumin -- CuATSM Cu-diacetyl-bis (N4-methylthiosemicarbazone) -- d days -- DAB 3, 3′-diaminobenzidine -- h hours -- Het hydroethidine -- Keap1 Kelch-like ECH-associated protein 1 -- M.O.M mouse on mouse -- MCA middle cerebral artery -- Nrf2 nuclear erythroid 2-related factor 2 -- OKD48 Keap1-dependent oxidative stress detector No.48 -- PCR polymerase chain reaction -- ROI sregions of interest -- Tmcao transient middle cerebral artery occlusion; CuATSM -- Edaravone -- Oxidative stress -- Stroke -- Transient middle cerebral artery occlusion; Neurosciences -- Research -- Periodicals; Neurosciences -- Research -- Japan -- Periodicals; Neurology -- Periodicals; Neurosciences -- Periodicals; Neurosciences -- Recherche -- Périodiques; Neurosciences -- Recherche -- Japon -- Périodiques; Neurosciences -- Research; Japan; Periodicals; 612.8
• DOI: 10.1016/j.neures.2020.05.009
• Languages: English
• ISSNs: 0168-0102

Abstract

Highlights: The anti-oxidative stress treatment is a crucial therapeutic strategy for ischemic stroke. CuATSM showed strong antioxidative and neuroprotective effects in acute ischemic stroke. These effects was enhanced by the combination with edaravone. The combination therapy of CuATSM and edaravone may provide a new effective therapy for acute ischemic stroke patients in the future. Abstract: Cu-diacetyl-bis (N4-methylthiosemicarbazone) (CuATSM) has both anti-oxidative and anti-inflammatory activities, but its therapeutic efficacy for oxidative stress has not been thoroughly investigated in acute ischemic stroke. Here, the present study was designed to assess the efficacies of CuATSM in acute ischemic stroke by comparing with the standard neuroprotective reagent edaravone. Mice were subjected to transient middle cerebral occlusion (tMCAO) for 60 min, and then intravenously administrated with CuATSM (1.5 mg/kg) or edaravone (3 mg/kg) just after the reperfusion, and examined at 1 and 3 d. Compared with the vehicle group, CuATSM treatment decreased infarct volumes and oxidative stress at 3d after tMCAO, which was further enhanced by combined CuATSM + edaravone treatment as compared with single CuATSM group, but not improve neurobehaviors. The present study demonstrated that CuATSM showed strong antioxidative and neuroprotective effects in acute ischemic stroke, which was enhanced by the combination with edaravone.