Dibutyl phthalate exposure disrupts the progression of meiotic prophase I by interfering with homologous recombination in fetal mouse oocytes. (September 2019)

• Record Type: Journal Article
• Authors: Tu, Zhihan; Mu, Xinyi; Chen, Xuemei; Geng, Yanqing; Zhang, Yan; Li, Qingying; Gao, Rufei; Liu, Taihang; Wang, Yingxiong; He, Junlin
• Is Part Of: Environmental pollution. Volume 252(2019):Part A
• Page Start: 388
• Page End: 398
• Publication Date: 2019-09
• Subjects: Dibutyl phthalate -- Meiotic prophase I -- Homologous recombination -- Oogenesis; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2019.05.107
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Dibutyl phthalate (DBP), one of the most widely used plasticizers, is a known environmental endocrine disruptor that impairs male and female fertility. In this study, oral administration of DBP was given to pregnant mice on 14.5 days post coitus (dpc) for 3 days; and additionally, DBP was added into the culture of 14.5 dpc fetal ovaries for 3 days. DBP exposure during gestation disturbed the progression of meiotic prophase I of mouse oocytes, specifically from the zygotene to pachytene stages. Meanwhile, the DBP-exposed pachytene oocytes showed increased homologous recombination sites and unrepaired DNA damage. Furthermore, DBP caused DNA damage by increasing oxidative stress, decreased the expression of multiple critical meiotic regulators, and consequently induced oocyte apoptosis. Moreover, the effect of DBP on meiosis I prophase involved estrogen receptors α and β. Collectively, these results demonstrated a set of meiotic defects in DBP-exposed fetal oocytes. As aberrations in homologous recombination can result in aneuploid gametes and embryos, this study provides new support for the deleterious effects of phthalates. Graphical abstract: Image 1 Highlights: Fetal exposure of DBP inhibits the progression of meiotic prophase I in oocytes. Fetal exposure of DBP increases Homologous Recombination crossovers and DNA damage. DBP induces oxidative stress and apoptosis in fetal ovary. Effect of DBP on meiotic prophase I is mediated by Estrogen receptors.