Highly efficient magnetic labelling allows MRI tracking of the homing of stem cell‐derived extracellular vesicles following systemic delivery. Issue 3 (15th January 2021)

• Record Type: Journal Article
• Authors: Han, Zheng; Liu, Senquan; Pei, Yigang; Ding, Zheng; Li, Yuguo; Wang, Xinge; Zhan, Daqian; Xia, Shuli; Driedonks, Tom; Witwer, Kenneth W.; Weiss, Robert G.; van Zijl, Peter C.M.; Bulte, Jeff W.M.; Cheng, Linzhao; Liu, Guanshu
• Is Part Of: Journal of extracellular vesicles. Volume 10:Issue 3(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-01-15
• Subjects: acute kidney injury -- extracellular vesicle -- iPSC -- MRI -- myocardial injury -- stem cell; Cells -- Mechanical properties -- Periodicals; Transport Vesicles; Cells -- Mechanical properties; Periodicals; Fulltext; Internet Resources; Periodicals; Electronic journals; Periodicals; 571.63
• DOI: 10.1002/jev2.12054
• Languages: English
• ISSNs: 2001-3078

Abstract

Abstract: Human stem‐cell‐derived extracellular vesicles (EVs) are currently being investigated for cell‐free therapy in regenerative medicine applications, but the lack of noninvasive imaging methods to track EV homing and uptake in injured tissues has limited the refinement and optimization of the approach. Here, we developed a new labelling strategy to prepare magnetic EVs (magneto‐EVs) allowing sensitive yet specific MRI tracking of systemically injected therapeutic EVs. This new labelling strategy relies on the use of 'sticky' magnetic particles, namely superparamagnetic iron oxide (SPIO) nanoparticles coated with polyhistidine tags, to efficiently separate magneto‐EVs from unencapsulated SPIO particles. Using this method, we prepared pluripotent stem cell (iPSC)‐derived magneto‐EVs and subsequently used MRI to track their homing in different animal models of kidney injury and myocardial ischemia. Our results showed that iPSC‐derived EVs preferentially accumulated in the injury sites and conferred substantial protection. Our study paves a new pathway for preparing highly purified magnetic EVs and tracking them using MRI towards optimized, systemically administered EV‐based cell‐free therapies.