Deoxynivalenol induces apoptosis and disrupts cellular homeostasis through MAPK signaling pathways in bovine mammary epithelial cells. (September 2019)

• Record Type: Journal Article
• Authors: Lee, Jin-Young; Lim, Whasun; Park, Sunwoo; Kim, Jinyoung; You, Seungkwon; Song, Gwonhwa
• Is Part Of: Environmental pollution. Volume 252(2019):Part A
• Page Start: 879
• Page End: 887
• Publication Date: 2019-09
• Subjects: Deoxynivalenol -- Mammary -- Cow -- Apoptosis -- Signaling transduction; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2019.06.001
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Deoxynivalenol (DON), a fungus-derived mycotoxin, also known as vomitoxin, is found in a wide range of cereal grains and grain-based food products. The biological toxicity of DON has been described in various species, but its toxicity and functional effects in mammary epithelial cells are unclear. In this study, we investigated the effect of DON on bovine mammary epithelial (MAC-T) cells using mechanistic approaches. We detected DON-induced cell cycle abrogation and calcium deficiency, leading to apoptotic cell death via MAPK signaling pathways. Moreover, we studied the transcriptional activation of blood and milk junctional regulators as well as inflammatory cytokines in response to DON. The results of this study contribute to a comprehensive understanding of DON-associated toxicity mechanisms in bovine mammary epithelial cells, which may facilitate the enhancement of milk stabilization in parallel with the establishment of safety profiles to protect against DON contamination in livestock farms and in the food industry. Graphical abstract: Hypothetical schematic illustration of the effects of deoxynivalenol in bovine mammary epithelial cells. Deoxynivalenol activates the MAPK pathway, induces DNA fragmentation, and inhibits cell cycle progression in bovine mammary epithelial cells. Furthermore, deoxynivalenol decreases both cytosolic and mitochondrial Ca2+ concentrations and alters the gene expression levels of occludin and claudin-3 and the composition of tight junction proteins in MAC-T cells. Moreover, deoxynivalenol increases the mRNA levels of inflammatory cytokines. Image 1 Highlights: Deoxynivalenol induces cell cycle arrest and apoptosis of mammary epithelial cells. Deoxynivalenol interferes intracellular calcium homeostasis of mammary epithelia. Deoxynivalenol disrupts mitochondrial membrane potential of mammary epithelia. Deoxynivalenol contamination may affect mammary functions of lactating cows. Abstract : Deoxynivalenol contamination induces cell cycle arrest and apoptosis in bovine mammary epithelial cells via MAPK signaling pathways.