Roundup® confers cytotoxicity through DNA damage and Mitochondria-Associated apoptosis induction. (September 2019)

• Record Type: Journal Article
• Authors: Hao, Youwu; Chen, Hui; Xu, Wenping; Gao, Jufang; Yang, Yun; Zhang, Yang; Tao, Liming
• Is Part Of: Environmental pollution. Volume 252(2019):Part A
• Page Start: 917
• Page End: 923
• Publication Date: 2019-09
• Subjects: Glyphosate-based herbicide -- Cytotoxicity -- DNA damage -- Mitochondria-associated apoptosis -- Human health risk; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2019.05.128
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Glyphosate-based herbicides (GBH) are the most widely used pesticides in the world. The extensive use of them increases the potential human health risk, including the human inhalation toxicity risk. We studied the effect of the most famous GBH Roundup® (RDP) in the concentration range from 50 to 125 μg/mL on Mitochondria-Associated apoptosis and DNA damage in Human alveolar carcinoma cells (A549 cells). Alkaline comet assay, immunofluorescence assay and Flow Cytometric Analysis assay were employed to detect DNA damages and apoptosis of A549 cells. We found RDP caused concentration-dependent increases in DNA damages and proportion of apoptotic cells in A549 cells. RDP induced the DNA single-strand breaks and double-strand breaks; the collapse of mitochondrial membrane by increasing Bax/Bcl-2, resulting in the release of cytochrome c into cytosol and then activated caspase-9/-3, cleaved poly (ADP-ribose) polymerase (PARP) in human lung tissue cells. The results demonstrate that RDP can induce A549 cells cytotoxic effects in vitro at the concentration lower than the occupational exposures level of workers, which means RDP has a potential threat to human health. Graphical abstract: Image 10185 Highlights: The cytotoxicity of commercial formulations of herbicides was investigated. Roundup® induces DNA damage in human A549 cells. Roundup® induces apoptotic cell death in human A549 cells. Attracting attention on the human health risk which induced by pesticide formulation.