MiR-375 Promotes Pancreatic Differentiation In Vitro by Affecting Different Target Genes at Different Stages. (9th April 2021)

• Record Type: Journal Article
• Authors: Lu, Zhenyu; Wang, Jing; Wang, Xu; Li, Zhiying; Niu, Dan; Wang, Min; Xiang, Jinzhu; Yue, Yongli; Xia, Yajuan; Li, Xueling
• Other Names: Zhang Dunfang Academic Editor.
• Is Part Of: Stem cells international. Volume 2021(2021)
• Publication Date: 2021-04-09
• Subjects: Stem Cells -- Periodicals; Stem Cells -- Therapeutic use -- Periodicals; Stem Cells -- Transplantation -- Periodicals; 616.0277405
• DOI: 10.1155/2021/6642983
• Languages: English
• ISSNs: 1687-966X

Abstract

Abstract : Human embryonic stem cells (hESCs) possess the ability to differentiate into insulin-producing cells (IPCs), which can be used to treat type I diabetes. miR-375 is an essential transcriptional regulator in the development and maturation of the pancreas. In this study, we optimized a protocol to differentiate hESCs into IPCs and successfully obtained IPCs. Then, we performed overexpression and inhibition experiments of miR-375 on cells at different stages of differentiation and performed RNA-seq. The results showed that the expression of miR-375 fluctuated during hESC differentiation and was affected by miR-375 mimics and inhibitors. miR-375 influences global gene expression and the target genes of miR-375. The overexpression of miR-375 can cause changes in multiple signaling pathways during pancreatic development. miR-375 is a major participant in the differentiation of pancreatic β -cells through different target genes at different stages. This study provides new ideas for further investigation of how microRNAs affect cell fate and gene transcription.