One step carboxyl group isotopic labeling for quantitative analysis of intact N-glycopeptides by mass spectrometry. Issue 34 (29th March 2021)

• Record Type: Journal Article
• Authors: Sun, Zhenyu; Ji, Guanghui; Wang, Guoli; Wei, Lei; Zhang, Ying; Lu, Haojie
• Is Part Of: Chemical communications. Volume 57:Issue 34(2021)
• Page Start: 4154
• Page End: 4157
• Publication Date: 2021-03-29
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d1cc00197c
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : One-step carboxyl group isotopic labeling for quantitative analysis of intact N -glycopeptides. Abstract : Here, we have developed an approach termed methylamine stable isotope labeling (MeSIL) to relatively quantify N -glycopeptides through one step labeling. It is the first time that this approach is applied to measure N -glycopeptide changes in huh7 cells after Zika virus infection and it has been revealed that differentially expressed N -glycopeptides played important roles in virus infection at the glycosylation site-specific level.