Comparative evaluation and design of a G-triplex/thioflavin T-based molecular beacon. Issue 8 (25th March 2021)

• Record Type: Journal Article
• Authors: Gao, Jingru; Liu, Qiang; Liu, Wei; Jin, Yan; Li, Baoxin
• Is Part Of: Analyst. Volume 146:Issue 8(2021)
• Page Start: 2567
• Page End: 2573
• Publication Date: 2021-03-25
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/d1an00252j
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : Compared with the common G-quadruplex-based molecular beacon, the G-triplex-based molecular beacon exhibits higher sensitivity and faster response speed. Abstract : Both G-quadruplex (G4) and G-triplex (G3) can bind thioflavin T (ThT) to light up the fluorescence of ThT. G4/ThT and G3/ThT can be used as fluorescent indicators to construct a label-free molecular beacon (MB). In this work, we present a comparative perspective of G3/ThT-based MB and G4/ThT-based MB. The results showed that the G3/ThT-based MB had higher sensitivity and faster response speed than the G4/ThT-based MB. Furthermore, we systematically studied the effect of stem length and varying pairs on the response of the G3/ThT-based MB, and then proposed one rational design of the G3/ThT-based MB. This work demonstrates that the shorter G3 is more suitable for constructing the MB stem. This present work opens a promising way to develop a sensitive, simple and homogeneous biosensing method.