Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition). Issue 10 (21st October 2019)

• Record Type: Journal Article
• Authors: Cossarizza, Andrea; Chang, Hyun‐Dong; Radbruch, Andreas; Acs, Andreas; Adam, Dieter; Adam‐Klages, Sabine; Agace, William W.; Aghaeepour, Nima; Akdis, Mübeccel; Allez, Matthieu; Almeida, Larissa Nogueira; Alvisi, Giorgia; Anderson, Graham; Andrä, Immanuel; Annunziato, Francesco; Anselmo, Achille; Bacher, Petra; Baldari, Cosima T.; Bari, Sudipto; Barnaba, Vincenzo; Barros‐Martins, Joana; Battistini, Luca; Bauer, Wolfgang; Baumgart, Sabine; Baumgarth, Nicole; Baumjohann, Dirk; Baying, Bianka; Bebawy, Mary; Becher, Burkhard; Beisker, Wolfgang; Benes, Vladimir; Beyaert, Rudi; Blanco, Alfonso; Boardman, Dominic A.; Bogdan, Christian; Borger, Jessica G.; Borsellino, Giovanna; Boulais, Philip E.; Bradford, Jolene A.; Brenner, Dirk; Brinkman, Ryan R.; Brooks, Anna E. S.; Busch, Dirk H.; Büscher, Martin; Bushnell, Timothy P.; Calzetti, Federica; Cameron, Garth; Cammarata, Ilenia; Cao, Xuetao; Cardell, Susanna L.; Casola, Stefano; Cassatella, Marco A.; Cavani, Andrea; Celada, Antonio; Chatenoud, Lucienne; Chattopadhyay, Pratip K.; Chow, Sue; Christakou, Eleni; Čičin‐Šain, Luka; Clerici, Mario; Colombo, Federico S.; Cook, Laura; Cooke, Anne; Cooper, Andrea M.; Corbett, Alexandra J.; Cosma, Antonio; Cosmi, Lorenzo; Coulie, Pierre G.; Cumano, Ana; Cvetkovic, Ljiljana; Dang, Van Duc; Dang‐Heine, Chantip; Davey, Martin S.; Davies, Derek; De Biasi, Sara; Del Zotto, Genny; Dela Cruz, Gelo Victoriano; Delacher, Michael; Della Bella, Silvia; Dellabona, Paolo; Deniz, Günnur; Dessing, Mark; Di Santo, James P.; Diefenbach, Andreas; Dieli, Francesco; Dolf, Andreas; Dörner, Thomas; Dress, Regine J.; Dudziak, Diana; Dustin, Michael; Dutertre, Charles‐Antoine; Ebner, Friederike; Eckle, Sidonia B. G.; Edinger, Matthias; Eede, Pascale; Ehrhardt, Götz R.A.; Eich, Marcus; Engel, Pablo; Engelhardt, Britta; Erdei, Anna; Esser, Charlotte; Everts, Bart; Evrard, Maximilien; Falk, Christine S.; Fehniger, Todd A.; Felipo‐Benavent, Mar; Ferry, Helen; Feuerer, Markus; Filby, Andrew; Filkor, Kata; Fillatreau, Simon; Follo, Marie; Förster, Irmgard; Foster, John; Foulds, Gemma A.; Frehse, Britta; Frenette, Paul S.; Frischbutter, Stefan; Fritzsche, Wolfgang; Galbraith, David W.; Gangaev, Anastasia; Garbi, Natalio; Gaudilliere, Brice; Gazzinelli, Ricardo T.; Geginat, Jens; Gerner, Wilhelm; Gherardin, Nicholas A.; Ghoreschi, Kamran; Gibellini, Lara; Ginhoux, Florent; Goda, Keisuke; Godfrey, Dale I.; Goettlinger, Christoph; González‐Navajas, Jose M.; Goodyear, Carl S.; Gori, Andrea; Grogan, Jane L.; Grummitt, Daryl; Grützkau, Andreas; Haftmann, Claudia; Hahn, Jonas; Hammad, Hamida; Hämmerling, Günter; Hansmann, Leo; Hansson, Goran; Harpur, Christopher M.; Hartmann, Susanne; Hauser, Andrea; Hauser, Anja E.; Haviland, David L.; Hedley, David; Hernández, Daniela C.; Herrera, Guadalupe; Herrmann, Martin; Hess, Christoph; Höfer, Thomas; Hoffmann, Petra; Hogquist, Kristin; Holland, Tristan; Höllt, Thomas; Holmdahl, Rikard; Hombrink, Pleun; Houston, Jessica P.; Hoyer, Bimba F.; Huang, Bo; Huang, Fang‐Ping; Huber, Johanna E.; Huehn, Jochen; Hundemer, Michael; Hunter, Christopher A.; Hwang, William Y. K.; Iannone, Anna; Ingelfinger, Florian; Ivison, Sabine M; Jäck, Hans‐Martin; Jani, Peter K.; Jávega, Beatriz; Jonjic, Stipan; Kaiser, Toralf; Kalina, Tomas; Kamradt, Thomas; Kaufmann, Stefan H. E.; Keller, Baerbel; Ketelaars, Steven L. C.; Khalilnezhad, Ahad; Khan, Srijit; Kisielow, Jan; Klenerman, Paul; Knopf, Jasmin; Koay, Hui‐Fern; Kobow, Katja; Kolls, Jay K.; Kong, Wan Ting; Kopf, Manfred; Korn, Thomas; Kriegsmann, Katharina; Kristyanto, Hendy; Kroneis, Thomas; Krueger, Andreas; Kühne, Jenny; Kukat, Christian; Kunkel, Désirée; Kunze‐Schumacher, Heike; Kurosaki, Tomohiro; Kurts, Christian; Kvistborg, Pia; Kwok, Immanuel; Landry, Jonathan; Lantz, Olivier; Lanuti, Paola; LaRosa, Francesca; Lehuen, Agnès; LeibundGut‐Landmann, Salomé; Leipold, Michael D.; Leung, Leslie Y.T.; Levings, Megan K.; Lino, Andreia C.; Liotta, Francesco; Litwin, Virginia; Liu, Yanling; Ljunggren, Hans‐Gustaf; Lohoff, Michael; Lombardi, Giovanna; Lopez, Lilly; López‐Botet, Miguel; Lovett‐Racke, Amy E.; Lubberts, Erik; Luche, Herve; Ludewig, Burkhard; Lugli, Enrico; Lunemann, Sebastian; Maecker, Holden T.; Maggi, Laura; Maguire, Orla; Mair, Florian; Mair, Kerstin H.; Mantovani, Alberto; Manz, Rudolf A.; Marshall, Aaron J.; Martínez‐Romero, Alicia; Martrus, Glòria; Marventano, Ivana; Maslinski, Wlodzimierz; Matarese, Giuseppe; Mattioli, Anna Vittoria; Maueröder, Christian; Mazzoni, Alessio; McCluskey, James; McGrath, Mairi; McGuire, Helen M.; McInnes, Iain B.; Mei, Henrik E.; Melchers, Fritz; Melzer, Susanne; Mielenz, Dirk; Miller, Stephen D.; Mills, Kingston H.G.; Minderman, Hans; Mjösberg, Jenny; Moore, Jonni; Moran, Barry; Moretta, Lorenzo; Mosmann, Tim R.; Müller, Susann; Multhoff, Gabriele; Muñoz, Luis Enrique; Münz, Christian; Nakayama, Toshinori; Nasi, Milena; Neumann, Katrin; Ng, Lai Guan; Niedobitek, Antonia; Nourshargh, Sussan; Núñez, Gabriel; O'Connor, José‐Enrique; Ochel, Aaron; Oja, Anna; Ordonez, Diana; Orfao, Alberto; Orlowski‐Oliver, Eva; Ouyang, Wenjun; Oxenius, Annette; Palankar, Raghavendra; Panse, Isabel; Pattanapanyasat, Kovit; Paulsen, Malte; Pavlinic, Dinko; Penter, Livius; Peterson, Pärt; Peth, Christian; Petriz, Jordi; Piancone, Federica; Pickl, Winfried F.; Piconese, Silvia; Pinti, Marcello; Pockley, A. Graham; Podolska, Malgorzata Justyna; Poon, Zhiyong; Pracht, Katharina; Prinz, Immo; Pucillo, Carlo E. M.; Quataert, Sally A.; Quatrini, Linda; Quinn, Kylie M.; Radbruch, Helena; Radstake, Tim R. D. J.; Rahmig, Susann; Rahn, Hans‐Peter; Rajwa, Bartek; Ravichandran, Gevitha; Raz, Yotam; Rebhahn, Jonathan A.; Recktenwald, Diether; Reimer, Dorothea; Reis e Sousa, Caetano; Remmerswaal, Ester B.M.; Richter, Lisa; Rico, Laura G.; Riddell, Andy; Rieger, Aja M.; Robinson, J. Paul; Romagnani, Chiara; Rubartelli, Anna; Ruland, Jürgen; Saalmüller, Armin; Saeys, Yvan; Saito, Takashi; Sakaguchi, Shimon; Sala‐de‐Oyanguren, Francisco; Samstag, Yvonne; Sanderson, Sharon; Sandrock, Inga; Santoni, Angela; Sanz, Ramon Bellmàs; Saresella, Marina; Sautes‐Fridman, Catherine; Sawitzki, Birgit; Schadt, Linda; Scheffold, Alexander; Scherer, Hans U.; Schiemann, Matthias; Schildberg, Frank A.; Schimisky, Esther; Schlitzer, Andreas; Schlosser, Josephine; Schmid, Stephan; Schmitt, Steffen; Schober, Kilian; Schraivogel, Daniel; Schuh, Wolfgang; Schüler, Thomas; Schulte, Reiner; Schulz, Axel Ronald; Schulz, Sebastian R.; Scottá, Cristiano; Scott‐Algara, Daniel; Sester, David P.; Shankey, T. Vincent; Silva‐Santos, Bruno; Simon, Anna Katharina; Sitnik, Katarzyna M.; Sozzani, Silvano; Speiser, Daniel E.; Spidlen, Josef; Stahlberg, Anders; Stall, Alan M.; Stanley, Natalie; Stark, Regina; Stehle, Christina; Steinmetz, Tobit; Stockinger, Hannes; Takahama, Yousuke; Takeda, Kiyoshi; Tan, Leonard; Tárnok, Attila; Tiegs, Gisa; Toldi, Gergely; Tornack, Julia; Traggiai, Elisabetta; Trebak, Mohamed; Tree, Timothy I.M.; Trotter, Joe; Trowsdale, John; Tsoumakidou, Maria; Ulrich, Henning; Urbanczyk, Sophia; van de Veen, Willem; van den Broek, Maries; van der Pol, Edwin; Van Gassen, Sofie; Van Isterdael, Gert; van Lier, René A.W.; Veldhoen, Marc; Vento‐Asturias, Salvador; Vieira, Paulo; Voehringer, David; Volk, Hans‐Dieter; von Borstel, Anouk; von Volkmann, Konrad; Waisman, Ari; Walker, Rachael V.; Wallace, Paul K.; Wang, Sa A.; Wang, Xin M.; Ward, Michael D.; Ward‐Hartstonge, Kirsten A; Warnatz, Klaus; Warnes, Gary; Warth, Sarah; Waskow, Claudia; Watson, James V.; Watzl, Carsten; Wegener, Leonie; Weisenburger, Thomas; Wiedemann, Annika; Wienands, Jürgen; Wilharm, Anneke; Wilkinson, Robert John; Willimsky, Gerald; Wing, James B.; Winkelmann, Rieke; Winkler, Thomas H.; Wirz, Oliver F.; Wong, Alicia; Wurst, Peter; Yang, Jennie H. M.; Yang, Juhao; Yazdanbakhsh, Maria; Yu, Liping; Yue, Alice; Zhang, Hanlin; Zhao, Yi; Ziegler, Susanne Maria; Zielinski, Christina; Zimmermann, Jakob; Zychlinsky, Arturo;
• Is Part Of: European journal of immunology. Volume 49:Issue 10(2019)
• Page Start: 1457
• Page End: 1973
• Publication Date: 2019-10-21
• Subjects: Immunology -- Periodicals; 616.079
• DOI: 10.1002/eji.201970107
• Languages: English
• ISSNs: 0014-2980

Abstract

Abstract: These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer‐reviewed by leading experts in the field, making this an essential research companion.